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Description
Overview
Recognizes the ~42-44 kDa ERK1 and ERK2 proteins phosphorylated at Thr202 and Tyr204 in vanadate-treated A431 cells. Supplied with a control lysate.
Catalogue Number
442706
Brand Family
Calbiochem®
Synonyms
Anti-MAPK1/2, Activated
References
Product Information
Form
Lyophilized
Formulation
100 µg antibody lyophilized from 2X PBS, sucrose, polyethylene glycol (PEG) and 150 µl lyophilized control lysate from 1 x 10⁶ vanadate-treated A431 cells.
Antibody requires phosphorylation at both the Thr and Tyr site for recognition. Does not recognize non-phosphorylated MAP kinase 1/2. Does not cross-react with SAPK 1 or SAPK2 proteins. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
a synthetic phosphopeptide corresponding to amino acids surrounding the Thr204/204 phosphorylation stie of mouse ERK1 and ERK2
Immunogen
Human
Clone
12D4
Host
Mouse
Isotype
IgG₁
Species Reactivity
Human
Mouse
Rat
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Reconstitute antibody with 1 ml dH₂O. Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Reconstitute the positive control cell lysate with 200 µl dH₂O. Following complete solubilization add 200 µl 2X SDS-PAGE sample buffer and incubate at 90°C for 5 min. Aliquot and freeze (-20°C). Avoid freeze/thaw cycles of reconstituted antibody and control lysate.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the ~42-44 kDa ERK1 and ERK2 protein phosphorylated on Thr202/Tyr204. Supplied with control lysate consisting of 1 x 106 vanadate-treated A431 cells.
Host
Mouse
Immunogen species
Human
Immunogen
a synthetic phosphopeptide corresponding to amino acids surrounding the Thr204/204 phosphorylation stie of mouse ERK1 and ERK2
Clone
12D4
Isotype
IgG₁
Species
human, mouse, rat
Positive control
vanadate-treated A431 cells (supplied)
Form
Lyophilized
Formulation
100 µg antibody lyophilized from 2X PBS, sucrose, polyethylene glycol (PEG) and 150 µl lyophilized control lysate from 1 x 10⁶ vanadate-treated A431 cells.
Preservative
≤0.1% sodium azide (antibody only)
Comments
Antibody requires phosphorylation at both the Thr and Tyr site for recognition. Does not recognize non-phosphorylated MAP kinase 1/2. Does not cross-react with SAPK 1 or SAPK2 proteins. Variables associated with assay conditions will dictate the proper working dilution.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Reconstitute antibody with 1 ml dH₂O. Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Reconstitute the positive control cell lysate with 200 µl dH₂O. Following complete solubilization add 200 µl 2X SDS-PAGE sample buffer and incubate at 90°C for 5 min. Aliquot and freeze (-20°C). Avoid freeze/thaw cycles of reconstituted antibody and control lysate.
