420092 PhosphoDetect™ Anti-JAK1 (pTyr1022/1023) Rabbit pAb

420092
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      420092-10T
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          Plastic ampoule 10 t
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          Description
          OverviewRecognizes the ~130 kDa phosphorylated JAK1 in LIF-treated 3T3-L1 cells. Does not cross-react with other JAKs or related kinases.
          Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
          Catalogue Number420092
          Brand Family Calbiochem®
          Application Data
          Detection of mouse JAK1, phosphorylated on Tyr1022/1023, by immunoblotting. Samples: Whole cell lysate from 3T3L1 cells left unstimulated (lane 1) or stimulated with LIF (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-JAK1 (pTyr1022/1023) Rabbit pAb (Cat. No. 420092) (1:1000) pre-incubated without peptide (lanes 1,2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
          References
          ReferencesLiu, K.D., et al. 1997. Curr. Biol. 7, 817.
          Gauzzi, M.C., et al. 1996. J. Biol. Chem. 271, 20494.
          Product Information
          DeclarationManufactured by Biosource International.
          FormLiquid
          FormulationIn Dulbecco's PBS (without Ca2+ and Mg2+), 1 mg/ml BSA, 50% glycerol, pH 7.3.
          Positive control3T3L1 cells treated with LIF
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Application NotesImmunoblotting (1:1000)
          Application CommentsA ~110 kDa degradation product is also visible in some cell or tissue preparations. Does not cross-react with any other JAK proteins. Immunogen sequence is conserved in pig JAK1, but cross-reactivity has not been tested. When used at a 1:1000 dilution, there is sufficient antibody for 10 immunoblots at 10 ml/blot. Variables associated with assay conditions will dictate the optimal dilution.
          Biological Information
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹⁰²²and Tyr¹⁰²³ phosphorylation sites of human JAK1 (Janus Kinase 1)
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Mouse
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing intitial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          420092

          References

          Reference overview
          Liu, K.D., et al. 1997. Curr. Biol. 7, 817.
          Gauzzi, M.C., et al. 1996. J. Biol. Chem. 271, 20494.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision13-May-2013 JSW
          ApplicationImmunoblotting (1:1000)
          Application Data
          Detection of mouse JAK1, phosphorylated on Tyr1022/1023, by immunoblotting. Samples: Whole cell lysate from 3T3L1 cells left unstimulated (lane 1) or stimulated with LIF (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-JAK1 (pTyr1022/1023) Rabbit pAb (Cat. No. 420092) (1:1000) pre-incubated without peptide (lanes 1,2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
          DescriptionRabbit polyclonal antibody adsorbed with non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~130 kDa JAK1 protein phosphorylated on Tyr1022/1023.
          BackgroundIn response to cytokines or related factors (interferons, interleukins, etc.), JAKs are activated via phosphorylation at 2 adjacent tyrosine residues, as well as perhaps one or more serine residues. Activation of JAKs can lead to the phosphorylation of STAT (signal transducers and activators of transcription) proteins, which dimerize and translocate to the nucleus. JAK1 is required for signal transduction through IL-2, IL-4, IL-6, GM-CSF, and interferon receptors.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹⁰²²and Tyr¹⁰²³ phosphorylation sites of human JAK1 (Janus Kinase 1)
          IsotypeIgG
          Specieshuman, mouse, not rat
          Positive control3T3L1 cells treated with LIF
          FormLiquid
          FormulationIn Dulbecco's PBS (without Ca2+ and Mg2+), 1 mg/ml BSA, 50% glycerol, pH 7.3.
          Preservative≤0.1% sodium azide
          CommentsA ~110 kDa degradation product is also visible in some cell or tissue preparations. Does not cross-react with any other JAK proteins. Immunogen sequence is conserved in pig JAK1, but cross-reactivity has not been tested. When used at a 1:1000 dilution, there is sufficient antibody for 10 immunoblots at 10 ml/blot. Variables associated with assay conditions will dictate the optimal dilution.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing intitial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesLiu, K.D., et al. 1997. Curr. Biol. 7, 817.
          Gauzzi, M.C., et al. 1996. J. Biol. Chem. 271, 20494.