382159 | PhosphoDetect™ Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb

382159
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      View Pricing & Availability
      Click To Print This Page

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      Nem, Dr, H, T, Xn Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      382159-50UG
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service

          Plastic ampoule 50 μg
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewRecognizes the ~17 kDa histone H3 protein phosphorylated at Ser10.
          Catalogue Number382159
          Brand Family Calbiochem®
          Application Data
          Detection of human histone H3, phosphorylated on Ser10, by immunoprecipitation. Sample: Acid extracted proteins from HeLa cells. Primary antibody: PhosphoDetect™ Anti-Histone H3 (pSer10) (7-20) Rabbit pAb (Cat. No. 382159) (assay dependent). Detection: chemiluminescence.
          References
          ReferencesAjiro, K., et al. 1996. J. Biol. Chem. 271, 13197.
          Chadee, D.N., et al. 1995. J. Biol. Chem. 270, 20098.
          Mahadevan, L.C., et al. 1991. Cell 65, 775.
          Product Information
          FormLiquid
          FormulationIn 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.
          Positive controlAcid-extracted proteins from colcemid-arrested HeLa cells
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoprecipitation
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Immunohistochemistry
          Application NotesImmunoblotting (0.5 µg/ml)
          Immunocytochemistry (5 µg/ml)
          Immunohistochemistry (see comments)
          Immunoprecipitation (4 µg/10 µg acid-extracted proteins from colcemid-arrested HeLa cells)
          Application CommentsFor immunocytochemistry, colcemid-arrested HeLa cells were fixed with 1% paraformaldehyde. Also detects histone H3 in Tetrahymena micronuclei. The antibody has been reported to detect mitotic cells in Xenopus, Caenorhabditis elegans, and Drosophila embryos. Does not cross-react with unphosphorylated histone H3. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal working dilution.
          Biological Information
          Immunogena synthetic phosphopeptide [ARKpSTGGKAPRKQL(C)] corresponding to amino acids 7-20 surrounding the Ser¹⁰ phosphorylation site of human histone H3, conjugated to KLH
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Nematode
          • Drosophila
          • Human
          • Tetrahymena
          • Xenopus
          Antibody TypePolyclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          382159

          References

          Reference overview
          Ajiro, K., et al. 1996. J. Biol. Chem. 271, 13197.
          Chadee, D.N., et al. 1995. J. Biol. Chem. 270, 20098.
          Mahadevan, L.C., et al. 1991. Cell 65, 775.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision02-August-2007 RFH
          ApplicationImmunoblotting (0.5 µg/ml)
          Immunocytochemistry (5 µg/ml)
          Immunohistochemistry (see comments)
          Immunoprecipitation (4 µg/10 µg acid-extracted proteins from colcemid-arrested HeLa cells)
          Application Data
          Detection of human histone H3, phosphorylated on Ser10, by immunoprecipitation. Sample: Acid extracted proteins from HeLa cells. Primary antibody: PhosphoDetect™ Anti-Histone H3 (pSer10) (7-20) Rabbit pAb (Cat. No. 382159) (assay dependent). Detection: chemiluminescence.
          DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~17 kDa histone H3 protein phosphorylated at Ser10.
          BackgroundHistone H1 and H3 phosphorylations correlate with the process of chromosome condensation. H3 mRNA-positive cells are most often found in tissues with a high proliferation rate, such as basal cells of the skin and crypt cells of the intestine.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic phosphopeptide [ARKpSTGGKAPRKQL(C)] corresponding to amino acids 7-20 surrounding the Ser¹⁰ phosphorylation site of human histone H3, conjugated to KLH
          IsotypeIgG
          SpeciesC. elegans, Drosophila, Tetrahymena, Xenopus, human
          Positive controlAcid-extracted proteins from colcemid-arrested HeLa cells
          FormLiquid
          FormulationIn 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsFor immunocytochemistry, colcemid-arrested HeLa cells were fixed with 1% paraformaldehyde. Also detects histone H3 in Tetrahymena micronuclei. The antibody has been reported to detect mitotic cells in Xenopus, Caenorhabditis elegans, and Drosophila embryos. Does not cross-react with unphosphorylated histone H3. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesAjiro, K., et al. 1996. J. Biol. Chem. 271, 13197.
          Chadee, D.N., et al. 1995. J. Biol. Chem. 270, 20098.
          Mahadevan, L.C., et al. 1991. Cell 65, 775.

          Related Products & Applications

          Suggested Product

          Catalog Number Description  
          71296 PhosphoSafe™ Extraction Reagent Show Pricing & Availability

          Related Products By: Brand Facete

          Categories

          Life Science Research > Antibodies and Assays > Primary Antibodies