361527 | PhosphoDetect™ Anti-GSK3β (pSer⁹) Mouse mAb (2D3)

361527
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      361527-1SET
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      Stocked 
      Discontinued
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          Plastic ampoule 1 set
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          Description
          OverviewRecognizes the ~46 kDa GSK-3β phosphorylated at Ser9. Supplied with a control lysate derived from 2 x 106 EGF-treated HepG2 cells.
          Catalogue Number361527
          Brand Family Calbiochem®
          References
          Product Information
          FormLiquid
          Formulation100 µg antibody in PBS, PEG, sucrose, 50% glycerol and 200 µl lyophilized control lysate.
          Positive controlPervanadate-treated HepG2 (included)
          Preservative≤0.1% sodium azide (antibody only)
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Application NotesELISA (0.05 µg/ml)
          Immunoblotting (0.5 µg/ml)
          Application CommentsFor immunoblotting with the positive control, use 10 µl (chemiluminescence) or 20 µl (colorimetric) per mini-gel lane. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser⁹ phosphorylation site of human glycogen synthase kinase 3β
          ImmunogenHuman
          Clone2D3
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          • Mouse
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsReconstitute the positive control lysate with 200 µl H₂O. Following complete solubilization, add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          361527
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision14-September-2009 RFH
          ApplicationELISA (0.05 µg/ml)
          Immunoblotting (0.5 µg/ml)
          DescriptionMouse monoclonal antibody purified by thiophilic adsorption and size exclusion chromatography. Recognizes the ~46 kDa GSK-3β phosphorylated at Ser9. Supplied with a control lysate derived from 2 x 106 EGF-treated HepG2 cells.
          HostMouse
          Immunogen speciesHuman
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser⁹ phosphorylation site of human glycogen synthase kinase 3β
          Clone2D3
          IsotypeIgG₁
          Specieshuman, mouse
          Positive controlPervanadate-treated HepG2 (included)
          FormLiquid
          Formulation100 µg antibody in PBS, PEG, sucrose, 50% glycerol and 200 µl lyophilized control lysate.
          Preservative≤0.1% sodium azide (antibody only)
          CommentsFor immunoblotting with the positive control, use 10 µl (chemiluminescence) or 20 µl (colorimetric) per mini-gel lane. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsReconstitute the positive control lysate with 200 µl H₂O. Following complete solubilization, add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min.
          Toxicity Standard Handling

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          Categories

          Life Science Research > Antibodies and Assays > Primary Antibodies