PS1008 PhosphoDetect™ Anti-FAK (pTyr⁸⁶¹) Rabbit pAb

PS1008
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      Ch, H, M Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      PS1008-100UL
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      Stocked 
      Discontinued
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          Plastic ampoule 100 ul
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          Description
          OverviewRecognizes the ~125 kDa FAK protein phosphorylated at Tyr861 in primary chick embryo fibroblasts transfected with human FAK.
          Catalogue NumberPS1008
          Brand Family Calbiochem®
          SynonymsAnti-FADK1, Phospho-Specific (Tyr⁸⁶¹), Anti-pp125FAK, Phospho-Specific (Tyr⁸⁶¹), Anti-Protein Tyrosine Kinase 2, Phospho-Specific (Tyr⁸⁶¹), Anti-PTK2, Phospho-Specific (Tyr⁸⁶¹), FAK 1
          Application Data
          Detection of FAK, phosphorylated at Tyr861, by immunoblotting. Samples: Whole cell lysates from chicken embryo fibroblasts (CEF) expressing FAK and plated on fibronectin. Primary antibody: PhosphoDetect™ Anti-FAK (pTyr861) Rabbit pAb (Cat. No. PS1008) (1:1000) pre-incubated with phosphopeptide immunogen (lane 1), a generic phosphotyrosine-containing peptide (lane 2), non-phosphorylated peptide corresponding to the phosphopeptide immunogen (lane 3), or without peptide (lane 4). Detection: chemiluminescence.
          References
          ReferencesEliceiri, B.P., et al. 2002. J. Cell. Biol. 157, 149.
          Hauck, C.R., et al. 2002. J. Biol. Chem. 277, 12487.
          Lacalle, R.A., et al. 2002. J. Cell. Biol. 157, 277.
          Lim, Y., et al. 2002. J. Biol. Chem. 277, 12735.
          Melendez, J., et al. 2002. J. Biol. Chem. 227, 45203.
          Rigacci, S., et al. 2002. J. Biol. Chem. 277, 41631.
          Datta, A., et al. 2001. Mol. Cell. Biol. 21, 7295.
          Nakamura, K., et al. 2001. Oncogene 20, 2626.
          Rocic, P., et al. 2001. J. Biol. Chem. 276, 21902.
          Slack, J.K., et al. 2001. Oncogene 20, 1152.
          Sieg, D.J., et al. 2000. Nature Cell. Biol. 2, 249.
          Vial, D., et al. 2000. J. Biol. Chem. 275, 28269.
          Wennerberg, K., et al 2000. Mol. Cell. Biol. 20, 5758.
          Product Information
          FormLiquid
          FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50 % glycerol, 1 mg/ml BSA, pH 7.3 (±0.1).
          Positive controlChicken embryo fibroblasts (CEF) expressing FAK, plated on fibronectin
          Preservative≤0.1 sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunohistochemistry
          Application NotesImmunoblotting (1:1000)
          Immunohistochemistry (see comments)
          Application CommentsThe immunogen sequence is 100% conserved in rat and Xenopus FAK but cross-reactivity has not been tested. This antibody is also reported to work for immunohistochemistry. Antibody should be titrated for optimal use in individual systems.
          Biological Information
          Immunogena synthetic phosphopeptide surrounding the Tyr⁸⁶¹ phosphorylation site of human FAK
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Chicken
          • Human
          • Mouse
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Irritant
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          PhosphoDetect™ Anti-FAK (pTyr⁸⁶¹) Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          PhosphoDetect™ Anti-FAK (pTyr⁸⁶¹) Rabbit pAb Certificates of Analysis

          TitleLot Number
          PS1008

          References

          Reference overview
          Eliceiri, B.P., et al. 2002. J. Cell. Biol. 157, 149.
          Hauck, C.R., et al. 2002. J. Biol. Chem. 277, 12487.
          Lacalle, R.A., et al. 2002. J. Cell. Biol. 157, 277.
          Lim, Y., et al. 2002. J. Biol. Chem. 277, 12735.
          Melendez, J., et al. 2002. J. Biol. Chem. 227, 45203.
          Rigacci, S., et al. 2002. J. Biol. Chem. 277, 41631.
          Datta, A., et al. 2001. Mol. Cell. Biol. 21, 7295.
          Nakamura, K., et al. 2001. Oncogene 20, 2626.
          Rocic, P., et al. 2001. J. Biol. Chem. 276, 21902.
          Slack, J.K., et al. 2001. Oncogene 20, 1152.
          Sieg, D.J., et al. 2000. Nature Cell. Biol. 2, 249.
          Vial, D., et al. 2000. J. Biol. Chem. 275, 28269.
          Wennerberg, K., et al 2000. Mol. Cell. Biol. 20, 5758.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision16-October-2007 RFH
          SynonymsAnti-FADK1, Phospho-Specific (Tyr⁸⁶¹), Anti-pp125FAK, Phospho-Specific (Tyr⁸⁶¹), Anti-Protein Tyrosine Kinase 2, Phospho-Specific (Tyr⁸⁶¹), Anti-PTK2, Phospho-Specific (Tyr⁸⁶¹), FAK 1
          ApplicationImmunoblotting (1:1000)
          Immunohistochemistry (see comments)
          Application Data
          Detection of FAK, phosphorylated at Tyr861, by immunoblotting. Samples: Whole cell lysates from chicken embryo fibroblasts (CEF) expressing FAK and plated on fibronectin. Primary antibody: PhosphoDetect™ Anti-FAK (pTyr861) Rabbit pAb (Cat. No. PS1008) (1:1000) pre-incubated with phosphopeptide immunogen (lane 1), a generic phosphotyrosine-containing peptide (lane 2), non-phosphorylated peptide corresponding to the phosphopeptide immunogen (lane 3), or without peptide (lane 4). Detection: chemiluminescence.
          DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site and a generic phosphotyrosine-containing peptide, followed by immunoaffinity chromatography. Recognizes the ~125 kDa FAK protein phosphorylated at Tyr861.
          BackgroundFocal adhesion kinase (FAK) is a non-receptor protein tyrosine kinase that plays a key role in maintaining focal adhesion function and cell survival. It is phosphorylated following growth factor or integrin-mediated cell adhesion stimulation. FAK is localized to focal adhesions through its C-terminal focal adhesion targeting (FAT) domain that acts by binding to paxillin and talin that are associated with the cytoplasmic tails of integrins. Phosphorylation of FAK at Tyr861 by Src promotes this interaction between FAK and integrins.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic phosphopeptide surrounding the Tyr⁸⁶¹ phosphorylation site of human FAK
          IsotypeIgG
          Specieschicken, human, mouse
          Positive controlChicken embryo fibroblasts (CEF) expressing FAK, plated on fibronectin
          FormLiquid
          FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50 % glycerol, 1 mg/ml BSA, pH 7.3 (±0.1).
          Preservative≤0.1 sodium azide
          CommentsThe immunogen sequence is 100% conserved in rat and Xenopus FAK but cross-reactivity has not been tested. This antibody is also reported to work for immunohistochemistry. Antibody should be titrated for optimal use in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Irritant
          ReferencesEliceiri, B.P., et al. 2002. J. Cell. Biol. 157, 149.
          Hauck, C.R., et al. 2002. J. Biol. Chem. 277, 12487.
          Lacalle, R.A., et al. 2002. J. Cell. Biol. 157, 277.
          Lim, Y., et al. 2002. J. Biol. Chem. 277, 12735.
          Melendez, J., et al. 2002. J. Biol. Chem. 227, 45203.
          Rigacci, S., et al. 2002. J. Biol. Chem. 277, 41631.
          Datta, A., et al. 2001. Mol. Cell. Biol. 21, 7295.
          Nakamura, K., et al. 2001. Oncogene 20, 2626.
          Rocic, P., et al. 2001. J. Biol. Chem. 276, 21902.
          Slack, J.K., et al. 2001. Oncogene 20, 1152.
          Sieg, D.J., et al. 2000. Nature Cell. Biol. 2, 249.
          Vial, D., et al. 2000. J. Biol. Chem. 275, 28269.
          Wennerberg, K., et al 2000. Mol. Cell. Biol. 20, 5758.