341292 PhosphoDetect™ Anti-FAK (pTyr³⁹⁷) Rabbit pAb

341292
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      Ch, Dr, Fg, H, M, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      341292-10T
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          Plastic ampoule 10 t
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          Description
          OverviewRecognizes the ~125 kDa FAK protein when phosphorylated at Tyr397. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
          Catalogue Number341292
          Brand Family Calbiochem®
          SynonymsAnti-Focal Adhesion Kinase, Phospho-Specific (Tyr³⁹⁷)
          Application Data
          Detection of FAK, phosphorylated on Tyr397, by immunoblotting. Sample: Whole cell lysate from CEF cells expressing FAK and plated on fibronectin. Primary antibody: PhosphoDetect™ Anti-FAK (pTyr397) Rabbit pAb (Cat. No. 341292) (1:000) pre-incubated without peptide (lanes 1), non-phosphopeptide corresponding to the immunogen (lane 2), a generic phosphotyrosine-containing peptide (lane 3), phosphopeptide derived from the corresponding region of Pyk2 (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
          References
          ReferencesFornaro, M., et al. 2000. Mol. Biol. Cell 11, 2235.
          Taylor, J, et al. 2000. J. Biol. Chem. 275, 19250.
          Vial, D., et al. 2000. J. Biol. Chem. 275, 28269.
          Wennerberg, K., et al. 2000. Mol. Cell. Biol. 20, 5758.
          Ridyard, M.S., and Sanders, E.J. 1999. Anat. Embryol. (Berl.) 199, 1.
          Product Information
          FormLiquid
          FormulationIn Dulbecco's PBS (no Mg²+ and Ca²+), 1 mg/ml BSA, 50% glycerol, pH 7.3.
          Positive controlChicken embryo fibroblasts expressing FAK or NIH3T3 cells treated with PDGF.
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunohistochemistry
          Application NotesImmunoblotting (1:1000)
          Immunohistochemistry (see comments; heat pre-treatment required)
          Application CommentsAlthough the antibody was adsorbed against a phospho-peptide corresponding to Pyk2, some cross-reactivity may still be detected in cases where Pyk2 is overexpressed relative to FAK within the same cell system. This antibody has also been reported to work for immunohistochemistry; heat pre-treatment with citrate buffer, pH 6.0 is required. When diluted 1:1000 there is sufficient antibody for 10 miniblots at 10 ml per blot. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr³⁹⁷ phosphorylation site of human FAK
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Chicken
          • Drosophila
          • Frog
          • Human
          • Mouse
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          341292

          References

          Reference overview
          Fornaro, M., et al. 2000. Mol. Biol. Cell 11, 2235.
          Taylor, J, et al. 2000. J. Biol. Chem. 275, 19250.
          Vial, D., et al. 2000. J. Biol. Chem. 275, 28269.
          Wennerberg, K., et al. 2000. Mol. Cell. Biol. 20, 5758.
          Ridyard, M.S., and Sanders, E.J. 1999. Anat. Embryol. (Berl.) 199, 1.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision19-November-2008 JSW
          SynonymsAnti-Focal Adhesion Kinase, Phospho-Specific (Tyr³⁹⁷)
          ApplicationImmunoblotting (1:1000)
          Immunohistochemistry (see comments; heat pre-treatment required)
          Application Data
          Detection of FAK, phosphorylated on Tyr397, by immunoblotting. Sample: Whole cell lysate from CEF cells expressing FAK and plated on fibronectin. Primary antibody: PhosphoDetect™ Anti-FAK (pTyr397) Rabbit pAb (Cat. No. 341292) (1:000) pre-incubated without peptide (lanes 1), non-phosphopeptide corresponding to the immunogen (lane 2), a generic phosphotyrosine-containing peptide (lane 3), phosphopeptide derived from the corresponding region of Pyk2 (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
          DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~125 kDa FAK protein phosphorylated on Tyr397.
          BackgroundDiscovered as a substrate for Src, FAK acts as a key element of integrin signaling. FAK is a non-receptor tyrosine kinase that plays a central role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. The Tyr397 autophosphorylation site binds Src family SH2 and the p85 subunit of PI3-Kinase.
          HostRabbit
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr³⁹⁷ phosphorylation site of human FAK
          IsotypeIgG
          SpeciesDrosophila, chicken, frog, human, mouse, rat
          Positive controlChicken embryo fibroblasts expressing FAK or NIH3T3 cells treated with PDGF.
          FormLiquid
          FormulationIn Dulbecco's PBS (no Mg²+ and Ca²+), 1 mg/ml BSA, 50% glycerol, pH 7.3.
          Preservative≤0.1% sodium azide
          CommentsAlthough the antibody was adsorbed against a phospho-peptide corresponding to Pyk2, some cross-reactivity may still be detected in cases where Pyk2 is overexpressed relative to FAK within the same cell system. This antibody has also been reported to work for immunohistochemistry; heat pre-treatment with citrate buffer, pH 6.0 is required. When diluted 1:1000 there is sufficient antibody for 10 miniblots at 10 ml per blot. Antibody should be titrated for optimal results in individual systems.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesFornaro, M., et al. 2000. Mol. Biol. Cell 11, 2235.
          Taylor, J, et al. 2000. J. Biol. Chem. 275, 19250.
          Vial, D., et al. 2000. J. Biol. Chem. 275, 28269.
          Wennerberg, K., et al. 2000. Mol. Cell. Biol. 20, 5758.
          Ridyard, M.S., and Sanders, E.J. 1999. Anat. Embryol. (Berl.) 199, 1.

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