324864 PhosphoDetect™ Anti-EGFR (pTyr¹¹⁷³) Mouse mAb (9H2)

324864
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R M Monoclonal Antibody

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      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      324864-1SET
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          Description
          OverviewRecognizes EGFR phosphorylated at Tyr1173 in vanadate-treated HepG2 cells.
          A positive control is provided.
          Catalogue Number324864
          Brand Family Calbiochem®
          SynonymsAnti-Epidermal Growth Factor Receptor (pTyr¹¹⁷³)
          Application Data
          Detection of human EGFR, phosphorylated on Tyr1173, by immunoblotting. Samples: Whole cell extracts of control (lane 1), EGF stimulated (lane 2) or pervanadate treated (lane 3) A549 cells. Primary antibody: PhosphoDetect™ Anti-EGFR (pTyr1173) Mouse mAb (9H2) (Cat. No. 324864) (0.5 µg/ml). Detection: chemiluminescence.
          References
          Product Information
          FormLyophilized
          Formulation100 µg antibody lyophilized from 2X PBS, PEG, sucrose and 200 µl lyophilized control lysate.
          Positive controlVanadate-treated HepG2 cells (included)
          Preservative≤0.1% sodium azide (antibody only)
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunoprecipitation
          Application NotesELISA (0.05 µg/ml)
          Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
          Immunoprecipitation (1-10 µg/sample)
          Application CommentsDoes not cross-react with non-phosphorylated EGFR or with unrelated tyrosine-phosphorylated proteins. Use 0.5% BSA and 0.5% casein for blocking. For immunoblotting with the positive control lysate use 5 µl for chemiluminescence or 20 µl for colorimetric per mini-gel lane. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogena synthetic phosphopeptide (NAEpYLRV) corresponding to amino acids 1170-1176 of human EGFR
          ImmunogenHuman
          Clone9H2
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsReconstitute the antibody with 1 ml H₂O (room temperature, 15 min). Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions. Reconstitute the positive control lysate in 200 µl H₂O. Add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min, aliquot, and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          324864

          Brochure

          Title
          Growth Factors and Cytokines Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision01-August-2007 RFH
          SynonymsAnti-Epidermal Growth Factor Receptor (pTyr¹¹⁷³)
          ApplicationELISA (0.05 µg/ml)
          Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
          Immunoprecipitation (1-10 µg/sample)
          Application Data
          Detection of human EGFR, phosphorylated on Tyr1173, by immunoblotting. Samples: Whole cell extracts of control (lane 1), EGF stimulated (lane 2) or pervanadate treated (lane 3) A549 cells. Primary antibody: PhosphoDetect™ Anti-EGFR (pTyr1173) Mouse mAb (9H2) (Cat. No. 324864) (0.5 µg/ml). Detection: chemiluminescence.
          DescriptionMouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the ~170 kDa EGFR protein phosphorylated on Tyr1173. Supplied with a positive control lysate derived from 2 x 106 vanadate-treated HepG2 cells.
          BackgroundBinding of EGF to the extracellular domain of the EGF-receptor results in receptor dimerization and autophosphorylation on tyrosine residues. The major site of tyrosine phosphorylation is tyrosine 1173.
          HostMouse
          Immunogen speciesHuman
          Immunogena synthetic phosphopeptide (NAEpYLRV) corresponding to amino acids 1170-1176 of human EGFR
          Clone9H2
          IsotypeIgG₁
          Specieshuman, mouse, rat
          Positive controlVanadate-treated HepG2 cells (included)
          FormLyophilized
          Formulation100 µg antibody lyophilized from 2X PBS, PEG, sucrose and 200 µl lyophilized control lysate.
          Preservative≤0.1% sodium azide (antibody only)
          CommentsDoes not cross-react with non-phosphorylated EGFR or with unrelated tyrosine-phosphorylated proteins. Use 0.5% BSA and 0.5% casein for blocking. For immunoblotting with the positive control lysate use 5 µl for chemiluminescence or 20 µl for colorimetric per mini-gel lane. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsReconstitute the antibody with 1 ml H₂O (room temperature, 15 min). Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions. Reconstitute the positive control lysate in 200 µl H₂O. Add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min, aliquot, and freeze (-20°C).
          Toxicity Standard Handling

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          Categories

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