516560 | Pertussis Toxin, Bordetella pertussis - CAS 70323-44-3 - Calbiochem

516560
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      Overview

      Replacement Information

      Key Specifications Table

      CAS #
      70323-44-3

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      516560-50UG
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          50 μg
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          Description
          OverviewObtain a uniform suspension before use. A protein endotoxin that catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins Gi, Go, and Gt. Used in the study of adenylate cyclase regulation and the role of Gi proteins. Holotoxin activity is determined in a CHO cell assay.
          NOTE: This product is not activated, hence for use in cell-free systems, activation is required.
          Catalogue Number516560
          Brand Family Calbiochem®
          SynonymsIslet-activating Protein
          References
          ReferencesJoshi, S.A., et al. 1998. FEBS Lett. 441, 67.
          Fryer, M.W. 1992. Neurosci. Lett. 146, 84.
          Gierschik, P. 1992. Curr. Topics Microbiol. Immunol. 175, 69.
          Harada, J., et al. 1992. Eur. J. Pharmacol. 227, 301.
          Hausman, S.Z., and Burns, D.L. 1992. J. Biol. Chem. 267, 13735.
          Kaslow, H.R., and Burns, D.L., 1992. FASEB J. 6, 2684.
          Zhu, J., et al. 1992. J. Pharmacol. Exp. Therap. 263, 1479.
          Hewlett, E.L., et al. 1983. Infect. Immun. 40, 1198.
          Moss, J., et al. 1983. J. Biol. Chem. 258, 11879.
          Product Information
          CAS number70323-44-3
          ATP CompetitiveN
          FormLyophilized
          FormulationLyophilized from 50 mM NaCl, 10 mM sodium phosphate buffer, pH 7.0.
          ReversibleN
          Applications
          Biological Information
          Primary TargetGuanine nucleotide-binding regulatory proteins Gi, Go, and Gt
          PurityFive distinct bands by SDS-PAGE
          Physicochemical Information
          Cell permeableN
          ContaminantsAdenylate cyclase activity: ≤35 pmol/min/µg in the presence of calmodulin
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSXW5883750
          Safety Information
          R PhraseR: 23/24/25

          Toxic by inhalation, in contact with skin and if swallowed.
          S PhraseS: 36/37/39-45

          Wear suitable protective clothing, gloves and eye/face protection.
          In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Toxic
          Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
          Storage +2°C to +8°C
          Do not freeze Yes
          Special InstructionsFollowing reconstitution, refrigerate (4°C). For long-term storage in solution, reconstitute with a high ionic strength buffer, such as 100 mM sodium phosphate, 500 mM sodium chloride, pH 7.0. Stock solutions are stable for at least 6 months for use in CHO cell assays and for 1-2 months for use in non-cellular systems.
          Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          516560

          References

          Reference overview
          Joshi, S.A., et al. 1998. FEBS Lett. 441, 67.
          Fryer, M.W. 1992. Neurosci. Lett. 146, 84.
          Gierschik, P. 1992. Curr. Topics Microbiol. Immunol. 175, 69.
          Harada, J., et al. 1992. Eur. J. Pharmacol. 227, 301.
          Hausman, S.Z., and Burns, D.L. 1992. J. Biol. Chem. 267, 13735.
          Kaslow, H.R., and Burns, D.L., 1992. FASEB J. 6, 2684.
          Zhu, J., et al. 1992. J. Pharmacol. Exp. Therap. 263, 1479.
          Hewlett, E.L., et al. 1983. Infect. Immun. 40, 1198.
          Moss, J., et al. 1983. J. Biol. Chem. 258, 11879.

          Citations

          Title
        • Soonkyu Chung, et al. (2005) Conjugated linoleic acid promotes human adipocyte insulin resistance through NF-κB-dependent cytokine production. Journal of Biological Chemistry 280, 38445-38456.
        • Olga Pervushina, et al. (2004) Platelet factor 4/CXCL4 induces phagocytosis and the generation of reactive oxygen metabolites in mononuclear phagocytes independently of Gi protein activation or intracellular calcium transients. Journal of Immunology 173, 2060-2067.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision28-September-2007 RFH
          SynonymsIslet-activating Protein
          DescriptionA protein endotoxin that catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins Gi, Go, and GT. Affects glucose homeostasis, immune cell function, and the induction of leukocytosis. Used in the study of adenylate cyclase regulation and the role of Gi proteins. Holotoxin activity is determined in CHO cell assay. Note: this product is not activated. If used with an intact cell system, the cells will activate the toxin. If used in a cell-free system, activation may be achieved by the methods of either Kaslow, H.R., and Burns, D.L. 1992. and Moss, J. 1983.
          FormLyophilized
          FormulationLyophilized from 50 mM NaCl, 10 mM sodium phosphate buffer, pH 7.0.
          CAS number70323-44-3
          RTECSXW5883750
          PurityFive distinct bands by SDS-PAGE
          ContaminantsAdenylate cyclase activity: ≤35 pmol/min/µg in the presence of calmodulin
          SolubilityReconstitute with 500 µl H₂O. Obtain a uniform suspension before each use by scraping the walls of the tube and gently pipetting the solution to mix.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Special InstructionsFollowing reconstitution, refrigerate (4°C). For long-term storage in solution, reconstitute with a high ionic strength buffer, such as 100 mM sodium phosphate, 500 mM sodium chloride, pH 7.0. Stock solutions are stable for at least 6 months for use in CHO cell assays and for 1-2 months for use in non-cellular systems.
          Toxicity Toxic
          ReferencesJoshi, S.A., et al. 1998. FEBS Lett. 441, 67.
          Fryer, M.W. 1992. Neurosci. Lett. 146, 84.
          Gierschik, P. 1992. Curr. Topics Microbiol. Immunol. 175, 69.
          Harada, J., et al. 1992. Eur. J. Pharmacol. 227, 301.
          Hausman, S.Z., and Burns, D.L. 1992. J. Biol. Chem. 267, 13735.
          Kaslow, H.R., and Burns, D.L., 1992. FASEB J. 6, 2684.
          Zhu, J., et al. 1992. J. Pharmacol. Exp. Therap. 263, 1479.
          Hewlett, E.L., et al. 1983. Infect. Immun. 40, 1198.
          Moss, J., et al. 1983. J. Biol. Chem. 258, 11879.
          Citation
        • Soonkyu Chung, et al. (2005) Conjugated linoleic acid promotes human adipocyte insulin resistance through NF-κB-dependent cytokine production. Journal of Biological Chemistry 280, 38445-38456.
        • Olga Pervushina, et al. (2004) Platelet factor 4/CXCL4 induces phagocytosis and the generation of reactive oxygen metabolites in mononuclear phagocytes independently of Gi protein activation or intracellular calcium transients. Journal of Immunology 173, 2060-2067.
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          Categories

          Life Science Research > Inhibitors and Biochemicals > Biochemicals > Toxins