69078 Perfect Protein™ HRP Western Blot Kit

69078
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      69078-3
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          Plastic ampoule 25 blot
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          Description
          OverviewThe Perfect Protein™ HRP Western Blot Kit includes an HRP Conjugate in addition to the Perfect Protein™ Western Markers (Cat. No. 69959). For a version containing prestained proteins for tracking electrophoresis and Western transfer, please refer to our complete listing of Trail Mix™ Western Markers and Blot Kits.


          A.Two parallel blots were incubated with primary antibodies for an expressed protein, then incubated with Anti-Mouse IgG AP Conjugate and processed using chemiluminescent detection. The S-protein AP Conjugate was included with secondary antibody for the blot on the left. Lanes: 1, 5 µl Perfect Protein Western Markers; 2, 10 ng induced cell extract; 3, 1 µg uninduced cell extract.
          B.Blot contains the Perfect Protein Western Markers plus purified GUS expressed in insect cells incubated with a GUS-specific monoclonal antibody, then incubated with Anti-Mouse IgG HRP Conjugate and S-protein HRP Conjugate and detected with chemiluminescent HRP substrate. Lanes: 1, 5 µl Perfect Protein Western Markers; 2, 800 ng purified GUS protein.
          Catalogue Number69078
          Brand Family Novagen®
          Features and benefits• Detect markers simply by adding the S-protein (AP or HRP Conjugate) or His•Tag® Monoclonal Antibody to the same incubation used for sample detection.
          • S-protein conjugate (included in kits) does not interfere with antibodies or streptavidin detection.
          • Can be used with colorimetric and chemiluminescent AP or HRP substrates.
          • Recombinant, unmodified markers give sharp, accurately sized bands.
          • Markers are supplied at the working dilution in gel loading buffer; concentration optimized for Western detection.
          • Protein sizes are 15, 25, 35, 50, 75, 100, and 150 kDa.
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          FormLiquid
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          Ship Code Blue Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
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          Categories

          Life Science Research > Protein Detection and Quantification > Western Blotting > Western Blotting Kits and Reagents