69049 Pellet Paint® Co-Precipitant

69049
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      Overview

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      69049-3
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          Plastic ampoule 125 rxn
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          69049-4
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              Plastic ampoule 1000 rxn
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              69049-EA
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                  Glass bottle
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                  Description
                  OverviewPellet Paint® Co-Precipitant is a visible dye-labeled carrier formulated specifically for use in alcohol precipitation of nucleic acids (McCormick 1995, McCormick 1996). The two-minute precipitation uses just 2 µl per reaction and requires no low-temperature incubations or prolonged centrifugation. Both RNA and DNA are efficiently precipitated from even dilute solutions (2 ng/ml) and the pellet is easily located by its vivid pink color. The pellet can be easily followed during washing steps and prevents losses during handling.

                  Most applications of PCR benefit from a clean-up step in which primers and other reactants are removed and the target DNA is concentrated (Taggart 1998). Pellet Paint Co- Precipitant is ideal for this cleanup because the procedure is rapid, primers < 50 nt in length are efficiently removed, and the DNA is quantitatively recovered. Furthermore, it is easy to tell when the DNA has been completely resuspended following the precipitation step.

                  Pellet Paint Co-Precipitant is compatible with most molecular biology procedures and is free of contaminating nucleic acids and nucleolytic enzymes. Although it absorbs in the UV range, accurate spectrophotometric measurements of DNA or RNA samples are possible; the absorbance ratio (provided with each package) can be used as a correction factor when determining nucleic acid concentration (McCormick 1996). Pellet Paint Co-Precipitant is compatible with automatted Cy5® sequencers. Pellet Paint NF Co- Precipitant is recommended for use with PE Applied Biosystems automated sequencers.



                  Catalogue Number69049
                  Brand Family Novagen®
                  Features and benefits• Direct visualization and tracking of precipitated material
                  References
                  ReferencesMcCormick, M. 1995. inNovations 4a, 10. McCormick, M. 1996. inNovations 5, 10. Taggart, E. W., et al. 1998. J. Clin. Microbiol. 36, 3408.
                  Product Information
                  250 µl or 2 mlPellet Paint Co-Precipitant
                  1 ml or 8 ml3 M Sodium Acetate, pH 5.2
                  DeclarationThis product is covered by U.S. Patents 7,550,447, 7,144,713, EP Patent 853,680, Canadian Patent 2,231,501 owned by EMD Chemicals Inc. or its Affiliates
                  FormLiquid
                  Applications
                  Biological Information
                  Physicochemical Information
                  Dimensions
                  Materials Information
                  Toxicological Information
                  Safety Information according to GHS
                  Safety Information
                  Product Usage Statements
                  Storage and Shipping Information
                  Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
                  Toxicity Standard Handling
                  Storage -20°C
                  Do not freeze Ok to freeze
                  Packaging Information
                  Transport Information
                  Supplemental Information
                  Specifications

                  Documentation

                  Pellet Paint® Co-Precipitant SDS

                  Title

                  Safety Data Sheet (SDS) 

                  Pellet Paint® Co-Precipitant Certificates of Analysis

                  TitleLot Number
                  69049

                  References

                  Reference overview
                  McCormick, M. 1995. inNovations 4a, 10. McCormick, M. 1996. inNovations 5, 10. Taggart, E. W., et al. 1998. J. Clin. Microbiol. 36, 3408.

                  Citations

                  Title
                • Brooks, C.L., 2007. Journal of Biological Chemistry 282, 22804.
                • Buttner, M.P., et al. 2007. Applied and Enviornmental Microbiology 73, 3505.
                • Halling, R.E., et al. 2007. Mycologia 99, 310.
                • Quan, S., et al. 2007. Journal of Biological Chemistry 282, 28823.
                • Tsai, C.J., et al. 2007. Procedings of the National Academy of Science 104, 7851.
                • Nowak, J.E., et al. 2006. Bio/Techniques 40, 220.
                • Lee, Y., et al. 2005 Clinical Cancer Research 11, 1059.
                • Mori, T., et al. 2005. Journal of Clinical Oncology 23, 9351.
                • Pendleton, L.C., et al. 2005. Journal of Biological Chemistry 280, 24252.
                • Beischlag, T.V., et al. 2004. Journal of Biological Chemistry 279, 54620.
                • Xu, M., et al. 2002. Journal of Clinical Microbiology 40, 2626.
                • Taggart, E.W., et al. 1998. Journal of Clinical Microbiology 36, 3408.
                • User Protocols

                  Title
                  TB146 Pellet Paint® Co-Precipitant

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                  Categories

                  Life Science Research > Genomic Analysis > DNA Preparation & Cloning > DNA Purification > DNA/RNA Clean-Up