33-016 | PI 3-Kinase HTRF™ Assay; 385 wells

33-016
1 plate  Kit capacity: 384 wells
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      Overview

      Replacement Information
      Description
      Catalogue Number33-016
      Brand Family Upstate
      Trade Name
      • HTRF
      • Upstate
      DescriptionPI 3-Kinase HTRF™ Assay; 385 wells
      References
      Product Information
      Components
      • 4X Reaction Buffer
      • PIP2 (Substrate; 1mM stock)
      • Stop Reagent A
      • Stop Reagent B
      • DM A Detection Reagent
      • DM B Detection Reagent
      • DM C Detection Reagent
      Detection methodHTRF
      Applications
      ApplicationThe PI 3-Kinase HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format. This pack size has a screening capacity of 1 plate.
      Key Applications
      • Kinase Assay
      Biological Information
      Species Reactivity
      • Human
      • Mouse
      Analytes Available
      • PI 3-Kinase
      Entrez Gene Number
      Entrez Gene SummaryPhosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
      Gene Symbol
      • PIK3CA
      • MGC142163
      • PI3K
      • MGC142161
      • p110-alpha
      Target Sub-FamilyPI3K/PI4K
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42336 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
      SIZE: 1068 amino acids; 124284 Da
      SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity).
      DISEASE: SwissProt: P42336 # Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. & Defects in PIK3CA are associated with breast cancer [MIM:114480]. & Defects in PIK3CA are associated with epithelial ovarian cancer (EOC) [MIM:604370].
      SIMILARITY: SwissProt: P42336 ## Belongs to the PI3/PI4-kinase family. & Contains 1 C2 domain. & Contains 1 PI3K/PI4K domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 plate
      Material PackageKit capacity: 384 wells
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      PI3 Kinase 4-Step Assay Reagent 1-Plate Kit - 1793690 1793690
      PI3 Kinase 4-Step Assay Reagent 1-Plate Kit - 1867155 1867155
      PI3 Kinase 4-Step Assay Reagent 1-Plate Kit - 1872388 1872388
      PI3 Kinase 4-Step Assay Reagent 1-Plate Kit - 1876548 1876548
      PI3 Kinase 4-Step Assay Reagent 1-Plate Kit - 2035490 2035490
      PI3 Kinase 4-Step Assay Reagent 1-Plate Kit - 2045061 2045061
      PI3 Kinase 4-Step Assay Reagent 1-Plate Kit - 2045068 2045068
      PI3 Kinase 4-Step Assay Reagent 1-Plate Kit - 2055084 2055084
      PI3 Kinase 4-Step Assay Reagent 1-Plate Kit - 2055388 2055388
      PI3 Kinase 4-Step Assay Reagent 1-Plate Kit - 2055390 2055390

      References

      Reference overviewPub Med ID
      A drug targeting only p110? can block phosphoinositide 3-kinase signalling and tumour growth in certain cell types.
      Stephen Jamieson,Jack U Flanagan,Sharada Kolekar,Christina Buchanan,Jackie D Kendall,Woo-Jeong Lee,Gordon W Rewcastle,William A Denny,Ripudaman Singh,James Dickson,Bruce C Baguley,Peter R Shepherd
      The Biochemical journal  438  2011

      Show Abstract Full Text Article
      21668414 21668414

      Technical Info

      Title
      Unlocking the Mystery of Cancer: Do the Mutant p110α Subunits of PI3-Kinase Hold the Key?

      Data Sheet

      Title
      PI 3-Kinase HTRF Assay

      User Guides

      Title
      PI 3-Kinase (Class I) HTRF® Assay, 1 plate (384 wells)

      Related Products & Applications

      Alternative Packsize

      Catalog Number Description  
      33-017 PI 3-Kinase HTRF™ Assay; 1920 wells Show Pricing & Availability
      33-036 PI 3-Kinase HTRF™ Assay; 10,000 wells Show Pricing & Availability
      33-037 PI 3-Kinase HTRF™ Assay; 50,000 wells Show Pricing & Availability

      Accessories

      Catalog Number Description  
      14-781 PI3 Kinase (p110α(E545K)/p85α), Murine, 10 µg Show Pricing & Availability
      14-782 PI 3-Kinase (p110α(E542K)/p85α), Human, 10 µg Show Pricing & Availability
      14-783 PI3 Kinase (p110α(E545K)/p85α), 10 µg Show Pricing & Availability
      14-785 PI3 Kinase (p110α/p85α) murine, 10 µg Show Pricing & Availability
      14-786 PI3 Kinase (p110α/p65α) Murine, 10 µg Show Pricing & Availability
      14-787 PI3 Kinase (p110α (H1047R)/p85α) Murine, 10 µg Show Pricing & Availability
      14-788 PI3 Kinase (p110β/p85β) Murine, 10 µg Show Pricing & Availability
      14-790 PI3 Kinase (p110α/p65α), 10 µg Show Pricing & Availability

      Product Families

      Related Products By: Brand Facete

      Categories

      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Enzyme Assay Kits and Reagents
      Life Science Research > Protein Detection and Quantification > Activity Assays > Kinase Assays