521215 | PDGF, AA Homodimer, Human, Recombinant, E. coli

521215
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      Overview

      Replacement Information

      Key Specifications Table

      Purity
      ≥97% by SDS-PAGE

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      521215-10UG
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      Available
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          Plastic ampoule 10 μg
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          Description
          OverviewRecombinant, human platelet derived growth factor AA (disulfide-linked dimer of two A-chain monomers) expressed in E. coli. Amino acid sequence is the long form of mature human PDGF-A chain and is identical to that of the native human nucleotide sequence except for the addition of an N-terminal methionine group. Implicated in the differentiation of cells of the oligodendrocyte lineage.
          Catalogue Number521215
          Brand Family Calbiochem®
          SynonymsPre-B Cell Stimulation Factor/Stromal Cell-Derived Factor-βrhPDGF-AA
          References
          ReferencesButt, A.M., et al. 1997. J. Neurosci. Res. 48, 588.
          Westermark, B., and Heldin, C.-H. 1991. Cancer Res. 51, 5087.
          Betsholtz, C., et al. 1986. Nature 320, 695.
          Raines, E.W., et al. 1985. Methods Enzymol. 109, 749.
          Product Information
          FormLyophilized
          FormulationLyophilized from a sterile-filtered solution in 30% acetonitrile, 0.1% TFA.
          Applications
          Biological Information
          Biological activity1-3 ng/ml
          Purity≥97% by SDS-PAGE
          Physicochemical Information
          ContaminantsEndotoxin: ≤1 EU/µg PDGF-AA
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at 4°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          521215

          References

          Reference overview
          Butt, A.M., et al. 1997. J. Neurosci. Res. 48, 588.
          Westermark, B., and Heldin, C.-H. 1991. Cancer Res. 51, 5087.
          Betsholtz, C., et al. 1986. Nature 320, 695.
          Raines, E.W., et al. 1985. Methods Enzymol. 109, 749.

          Brochure

          Title
          Growth Factors and Cytokines Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision16-May-2008 RFH
          SynonymsPre-B Cell Stimulation Factor/Stromal Cell-Derived Factor-βrhPDGF-AA
          DescriptionRecombinant, human platelet derived growth factor AA (disulfide-linked dimer of two A-chain monomers) expressed in E. coli. Amino acid sequence is the long form of mature human PDGF-A chain and is identical to that deduced from the native human nucleotide sequence except for the addition of an N-terminal methionine group.
          FormLyophilized
          FormulationLyophilized from a sterile-filtered solution in 30% acetonitrile, 0.1% TFA.
          Purity≥97% by SDS-PAGE
          ContaminantsEndotoxin: ≤1 EU/µg PDGF-AA
          Biological activity1-3 ng/ml
          SolubilityReconstitute to a concentration ≥10 µg/ml with sterile 4 mM HCl containing at least 0.1% HSA or BSA.
          Storage ≤ -70°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at 4°C.
          Toxicity Standard Handling
          Merck USA index14, 7525
          ReferencesButt, A.M., et al. 1997. J. Neurosci. Res. 48, 588.
          Westermark, B., and Heldin, C.-H. 1991. Cancer Res. 51, 5087.
          Betsholtz, C., et al. 1986. Nature 320, 695.
          Raines, E.W., et al. 1985. Methods Enzymol. 109, 749.

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          Categories

          Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
          Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors