499260 | Osteopontin, His•Tag®, Mouse, Recombinant, Mouse

499260
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      499260-50UG
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          Plastic ampoule 50 μg
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          Description
          OverviewRecombinant, mouse osteopontin fused at the C-terminus to a HisTag® sequence and expressed in NSO-derived Leu17-Asn294 (Glu99Gly) murine myeloma cells. A secreted, highly acidic, Ca2+-binding, RGD-containing, phosphorylated glycoprotein. Chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. Has been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Plays a significant role in inducing granulomas of various origin. OPN is also involved in the migration of embryonic fibroblasts, macrophages, and metastatic cells.
          M.W. 65,000 and 30,000 under reducing conditions.
          Catalogue Number499260
          Brand Family Calbiochem®
          SynonymsrmOPN
          References
          ReferencesXie, Y., et al. 2001. Kidney Int. 60, 1645.
          Chiba, S., et al. 2000. Microbiol. Immunol. 44, 319.
          Miyazaki, Y., et al. 1990. J. Biol. Chem. 265, 14432.
          Product Information
          FormLyophilized
          FormulationLyophilized from 0.2 µm sterile-filtered PBS, 50 µg BSA/µg rmOPN.
          Applications
          Biological Information
          Biological activity~200 ng immobilized osteopontin/well will mediate the adhesion of ≥25% of the 293 cells plated at 10⁵ cells/well
          Purity≥95% by SDS-PAGE
          Physicochemical Information
          ContaminantsEndotoxins: ≤100 pg/µg rmOPN
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 month at 4°C and for up to 3 months at -70°C. Avoid freeze/thaw cycles of stock solutions.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          499260

          References

          Reference overview
          Xie, Y., et al. 2001. Kidney Int. 60, 1645.
          Chiba, S., et al. 2000. Microbiol. Immunol. 44, 319.
          Miyazaki, Y., et al. 1990. J. Biol. Chem. 265, 14432.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision10-June-2010 JSW
          SynonymsrmOPN
          DescriptionRecombinant, mouse osteopontin fused at the C-terminus to a HisTag® sequence and expressed in NSO-derived Leu17-Asn294 (Glu99Gly) murine myeloma cells. A secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein. Osteopontin is expressed in several cell types including macrophages, fibroblasts, epithelial cells, T cells and osteoclasts in response to activation by cytokines, growth factors or inflammatory mediators. Mouse OPN cDNA encodes a 294 amino acid protein that has a 16 amino acid predicted signal peptide, which is cleaved to generate 278 amino acid mature protein. The mature protein contains an integrin binding arginine-glycine-aspartic acid (RGD) sequence. Exhibits some renoprotective actions in renal injury, such as increasing tolerance to acute ischemia, inhibiting inducible nitric oxide synthase, and reducing cell peroxide levels.
          FormLyophilized
          FormulationLyophilized from 0.2 µm sterile-filtered PBS, 50 µg BSA/µg rmOPN.
          Purity≥95% by SDS-PAGE
          ContaminantsEndotoxins: ≤100 pg/µg rmOPN
          Biological activity~200 ng immobilized osteopontin/well will mediate the adhesion of ≥25% of the 293 cells plated at 10⁵ cells/well
          SolubilityReconstitute to ≥100 µg/ml with sterile PBS.
          Storage ≤ -70°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 month at 4°C and for up to 3 months at -70°C. Avoid freeze/thaw cycles of stock solutions.
          Toxicity Standard Handling
          ReferencesXie, Y., et al. 2001. Kidney Int. 60, 1645.
          Chiba, S., et al. 2000. Microbiol. Immunol. 44, 319.
          Miyazaki, Y., et al. 1990. J. Biol. Chem. 265, 14432.