2740-OP OmniPur® Bradford Method Protein Assay Kit - Calbiochem

2740-OP
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      Overview

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      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      2740-1KIT
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          Description
          OverviewContains: Bradford Reagent, 0.15 M Sodium Chloride, and 0.5 mg/ml BSA Solution.

          Specifications:
          BSA Solution BSA Content: 0.45-0.55mg/ml
          BSA Solution Protease: None detected
          Bradford Reagent Pattern: Passes test
          Bradford Reagent Protease: None detected
          Sodium Chloride Solution Concentration: 130-170 mmol/l





          Intended for laboratory and manufacturing use only. Not for drug, food, or household use.
          Catalogue Number2740-OP
          Brand Family Calbiochem®
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          Ship Code Ambient Temperature Only
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
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          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          2740-OP
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision22-September-2012 JSW
          DescriptionThe Bradford method is a rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding.
          Recommended reaction conditions

          Materials Supplied
          • 1 liter of Bradford reagent
          • 1.5 ml of BSA
          • 200 ml of Sodium Chloride solution

          Protocol
          1. Into 4 separate microcentrifuge tubes, aliquot 5, 10, 15 and 20 µl of 0.5 mg/ml BSA solution. Bring the volume of each to 100 µl with 0.15N NaCl.
          2. Into 1 tube, aliquot 100 µl 0.15N NaCl. This will serve as a blank.
          3. Add to each tube, 1 ml Bradford Reagent and vortex. Allow to stand at room temperature for 2 minutes.
          4. Determine A 595 nm using a 1 ml microcuvette. Generate a standard curve by plotting absorbance at 595 nm versus protein concentration.
          5. For the unknown sample, repeat steps 1-4 using the unknown in place of the BSA. Plot the A 595 nm and use the standard curve as a reference to determine the concentration of the unknown sample.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze