506075 | Novel Inducer of Death-1, NID-1 - Calbiochem

506075
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical Formula
      C₁₅H₁₄N₂O₃S

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      5.06075.0001
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          Glass bottle 25 mg
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          Description
          OverviewA cell-permeable, nitrothiophenylpropenamide compound that induces active, energy-dependent, and rapid non-apoptotic cell death (EC50 = 1.65 µM) in several transformed (DRD, RKO, H1299) and non-transformed (BJeH ) human cells, in a variety of cancer cell lines, and in mouse embryonic fibroblasts. The cell death appears to be non-necrotic, non-oxidative and is independent of DNA damage or cell proliferation. NID-1 induced cell death, although distinct from macroautophagy, does show PI 3-kinase suppression, ATG5-dependence, p62 degradation, and higher LC3 levels. Also, NID-1-induced death is independent of caspases, Bax and Bak and requires lysosomal activity and cytosolic vacuolization. Rescues mutant-huntingtin-induced cell death in a cell-based model of Huntington’s disease. Shown to increase cathepsin L levels, but does not affect cathepsins B, C, and H.
          Catalogue Number506075
          Brand Family Calbiochem®
          Synonyms(E)-N-(2-ethylphenyl)-3-(5-nitrothiophen-2-yl)acrylamide
          References
          ReferencesVarma, H., et al. 2013. Exp. Cell Res.319, 1759.
          Product Information
          FormYellow solid
          Hill FormulaC₁₅H₁₄N₂O₃S
          Chemical formulaC₁₅H₁₄N₂O₃S
          Structure formula ImageStructure formula Image
          Applications
          Biological Information
          Purity≥98% by HPLC
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          References

          Reference overview
          Varma, H., et al. 2013. Exp. Cell Res.319, 1759.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision31-October-2013 JSW
          Synonyms(E)-N-(2-ethylphenyl)-3-(5-nitrothiophen-2-yl)acrylamide
          DescriptionA cell-permeable, nitrothiophenylpropenamide compound that induces active, energy-dependent, and rapid non-apoptotic cell death (EC50 = 1.65 µM) in several transformed (DRD, RKO, H1299) and non-transformed (BJeH ) human cells, in a variety of cancer cell lines, and in mouse embryonic fibroblasts. The cell death appears to be non-necrotic, non-oxidative and is independent of DNA damage or cell proliferation. NID-1 induced cell death, although distinct from macroautophagy, does show PI 3-kinase suppression, ATG5-dependence, p62 degradation, and higher LC3 levels. Also, NID-1-induced death is independent of caspases, Bax and Bak and requires lysosomal activity and cytosolic vacuolization. Rescues mutant-huntingtin-induced cell death in a cell-based model of Huntington′s disease. Shown to increase cathepsin L levels, but does not affect cathepsins B, C, and H.
          FormYellow solid
          Intert gas (Yes/No) Packaged under inert gas
          Chemical formulaC₁₅H₁₄N₂O₃S
          Structure formulaStructure formula
          Purity≥98% by HPLC
          SolubilityDMSO (50 mg/ml)
          Storage +2°C to +8°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Toxicity Standard Handling
          ReferencesVarma, H., et al. 2013. Exp. Cell Res.319, 1759.