SCR019 Neural Stem Cell Marker Characterization Kit

SCR019
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      Overview

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      Key Applications
      ICC, Cult
      Description
      Catalogue NumberSCR019
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionNeural Stem Cell Marker Characterization Kit
      OverviewNeural Stem Cell Marker Characterization Kit

      Introduction:

      Neural stem cells are present in both the developing and adult nervous system of all mammals, including humans (Gage, 2000). They possess the remarkable capacity to self-renew and to differentiate along specific pathways to generate the vast array of neuronal and glial cell types of the central nervous system (CNS). Due to their therapeutic promise, considerable attention has been focused on identifying the sources of stem cells, the signals that regulate their proliferation and the specification of neural stem cells towards more differentiated cell lineages.

      Presently, neural stem cells are often identified based upon the presence of molecular markers that are correlated with the stem and/or progenitor state along with the absence of a more differentiated phenotype as assessed through marker analysis. To aid researchers in the accurate identification of neural stem cells, CHEMICON® presents the Neural Stem Cell Characterization Kit (Catalog Number SCR019).

      CHEMICON®'s Neural Stem Cell Characterization Kit (Catalog Number SCR019) contains two molecular markers, Nestin (Lendahl, U. et al., 1990) and Sox 2 (Graham, V. et al., 2003), that are frequently used to identify neural stem/progenitor cells along with more differentiated lineage markers including Map2ab for neurons, GFAP for astrocytes and O1 for oligodendrocytes. Mouse and rabbit immunoglobulins for the assessment of background staining are also included.

      All of the antibodies provided in the kit have been tested and optimized for use in immunocytochemistry on adult rat neural stem cells. We recommend that CHEMICON®'s Neural Stem Cell Characterization Kit be used in conjunction with differentiation assays that demonstrate multipotentiality of the starting cell population. Please look for these differentiation assays along with rat neural stem cells from CHEMICON® in the near future.

      For Research Use Only; not for use in diagnostic procedure.
      Materials Required but Not Delivered1. Neural stem cells and culture reagents

      2. Adult Rat Neural Stem Cell Expansion Medium (DMEM / F12, B27 Supplement, 2 mM L-Glutamine and 1X solution of penicillin, streptomycin and fungizone (PSF))

      3. Chamber slides

      4. Glass coverslips

      5. Phosphate-Buffered Saline (1X PBS)

      6. Fixative (e.g. 4% Paraformaldehyde in 1X PBS)

      7. Blocking Solution (5% normal donkey serum, 0.3% Triton X-100 in 1X PBS)

      8. Non-Permeable Blocking Solution (5% normal donkey serum in 1X PBS)

      9. Fluorescent-labeled secondary antibodies. Donkey anti-mouse IgG, Cy3 conjugated (Cat. No. AP192C) and donkey anti-rabbit IgG, Cy3 conjugated (Cat. No. AP182C) are recommended

      10. 4'-6-Diamidino-2-phenylindole (DAPI) / PBS solution

      11. Anti-fading mounting solution (DABCO/PVA)

      12. Hemacytometer

      13. Microscope
      References
      Product Information
      Components
      • Mouse anti-Nestin: (Catalog No. 2003602) One vial containing 50 μg monoclonal antibody. Store at 2° to 8°C.
      • Rabbit anti-Sox 2: (Catalog No. 2003600) One vial containing 20 μg affinity purified polyclonal antibody. Store at 2° to 8°C.
      • Mouse anti-MAP-2: (Catalog No. MAB3418-50UG) One vial containing 50 μg monoclonal antibody. Store at 2° to 8°C.
      • Rabbit anti-GFAP: (Catalog No. AB5804) One vial containing 50 μL of polyclonal rabbit serum. Store at -20 °C.
      • Mouse anti-Oligodendrocyte marker O1: (Catalog No. 2003601) One vial containing 20 μg IgM monoclonal antibody. Store at-20 °C.
      • Mouse IgM: (Catalog No. 2003599) One vial containing 50 μg purified mouse IgM control antibody. Store at 2° to 8°C.
      • Mouse IgG: (Catalog No. PP54-100UG) One vial containing 100 μg purified mouse IgG control antibody. Store at -20 °C.
      • Rabbit IgG: (Catalog No. PP64-100UG) One vial containing 100 μg purified rabbit IgG antibody. Store at -20 °C.
      Applications
      ApplicationThe Neural Stem Cell Characterization Kit contains two molecular markers, Nestin & Sox 2 that are frequently used to identify neural stem/progenitor cells.
      Key Applications
      • Immunocytochemistry
      • Stem Cell Culture
      Biological Information
      Stem Cell Type
      • Neural Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsWhen stored at the recommended storage conditions (refer to Kit Components), components are stable up to the expiration date. Do not expose to elevated temperatures. Discard any remaining reagents after the expiration date.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications