530838 NMD Inhibitor - Calbiochem

530838
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical Formula
      C₂₁H₂₅N₃O₄S

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      5.30838.0001
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          Glass bottle 10 mg
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          Description
          OverviewA cell-permeable tetrahydroquinoxalinyl-thiophenecarboxylate compound that inhibits nonsense-mediated mRNA decay (NMD) in a dose-dependent manner and enhances the stability of premature termination codon (PTC) mutated p53 mRNA in N417 and HDQP-1 cells. However, it does not affect the stability of wild-type p53 in U2OS cells. Shown to dock reversibly within a SMG7 pocket and disrupt SMG7-UPF1 interaction and prevent their complex formation. Acts synergistically with G418 (Cat. No. 345810; 200 µg/ml) to increase p53 expression in p53 PTC mutant cells leading to increase mRNA levels of p21, Bax, and PUMA. Does not display any toxicity (> 50 µM) in multiple cell lines and has no effect on proliferation and protein synthesis in transformed and non-transformed cells.

          Please note that the molecular weight for this compound is batch-specific due to variable water content.
          Catalogue Number530838
          Brand Family Calbiochem®
          SynonymsEthyl 2-(((6,7-dimethyl-3-oxo-1,2,3,4-tetrahydro-2-quinoxalinyl)acetyl)amino)-4,5-dimethyl-3-thiophenecarboxylate, Nonsense-Mediated mRNA Decay Inhibitor 14, NMDI-14
          References
          ReferencesMartin, L., et al. 2014. Cancer Res. 74, 3104.
          Product Information
          FormYellow powder
          Hill FormulaC₂₁H₂₅N₃O₄S
          Chemical formulaC₂₁H₂₅N₃O₄S
          ReversibleY
          Applications
          Biological Information
          Primary TargetSMG7
          Purity≥95% by HPLC
          Physicochemical Information
          Cell permeableY
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          NMD Inhibitor - Calbiochem SDS

          Title

          Safety Data Sheet (SDS) 

          References

          Reference overview
          Martin, L., et al. 2014. Cancer Res. 74, 3104.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision13-February-2019 JSW
          SynonymsEthyl 2-(((6,7-dimethyl-3-oxo-1,2,3,4-tetrahydro-2-quinoxalinyl)acetyl)amino)-4,5-dimethyl-3-thiophenecarboxylate, Nonsense-Mediated mRNA Decay Inhibitor 14, NMDI-14
          DescriptionA cell-permeable tetrahydroquinoxalinyl-thiophenecarboxylate compound that inhibits nonsense-mediated mRNA decay (NMD) in a dose-dependent manner and enhances the stability of premature termination codon (PTC) mutated p53 mRNA in N417 and HDQP-1 cells. However, it does not affect the stability of wild-type p53 in U2OS cells. Shown to dock reversibly within a SMG7 pocket and disrupt SMG7-UPF1 interaction and prevent their complex formation. Acts synergistically with G418 (Cat. No. 345810; 200 µg/ml) to increase p53 expression in p53 PTC mutant cells leading to increase mRNA levels of p21, Bax, and PUMA. Does not display any toxicity (> 50 µM) in multiple cell lines and has no effect on proliferation and protein synthesis in transformed and non-transformed cells.
          FormYellow powder
          Intert gas (Yes/No) Packaged under inert gas
          Chemical formulaC₂₁H₂₅N₃O₄S
          Purity≥95% by HPLC
          SolubilityDMSO (10 mg/ml). Use only fresh DMSO for reconstitution.
          Storage -20°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Toxicity Standard Handling
          ReferencesMartin, L., et al. 2014. Cancer Res. 74, 3104.