480450 | NFF-2 - Calbiochem

480450
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      480450-1MG
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          Plastic ampoule 1 mg
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          Description
          OverviewA quenched fluorescence substrate for MMPs. MMP-3 (stromelysin-1) hydrolyzes this substrate (kcat/Km = 5.9 x 104 M-1sec-1) at a rate 60 times faster than the hydrolysis by MMP-1 (interstitial collagenase). NFF-2 shows little discrimination between MMP-3, MMP-2 (gelatinase-A; kcat/Km = 5 x 104 M-1s-1), and MMP-9 (gelatinase-B; kcat/Km = 5.5 x 104 M-1s-1).
          Supplied as a trifluoroacetate salt.
          Catalogue Number480450
          Brand Family Calbiochem®
          SynonymsMCA-RPKPYANvaWMK(DNP)-NH₂
          References
          ReferencesNagase, H., et al. 1994. J. Biol. Chem. 269, 20952.
          Product Information
          ATP CompetitiveN
          FormLyophilized yellow solid
          FormulationSupplied as a trifluoroacetate salt.
          Hill FormulaC₇₉H₁₀₅N₁₉O₁₉S
          Chemical formulaC₇₉H₁₀₅N₁₉O₁₉S
          ReversibleN
          Applications
          Biological Information
          Primary TargetMatrix Metalloproteinases (MMPs)
          Primary Target IC<sub>50</sub>kcat/Km = 5.9 x 104 M-1sec-1 as substrates for MMP-3 (stromelysin-1)
          Purity≥98% by HPLC
          Physicochemical Information
          Cell permeableN
          Emission max.
          Excitation max.
          Peptide SequenceMCA-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(DNP)-NH₂ (DNP = 2,4-dinitrophenyl; Nva = L-norvaline)
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Protect from Light Protect from light
          Protect from Moisture Protect from moisture
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          480450

          References

          Reference overview
          Nagase, H., et al. 1994. J. Biol. Chem. 269, 20952.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision11-August-2008 RFH
          SynonymsMCA-RPKPYANvaWMK(DNP)-NH₂
          DescriptionA quenched fluorescent substrate for MMPs (excitation maximum: ~325 nm; emission maximum: ~393 nm). MMP-3 (stromelysin-1) hydrolyzes this substrate (kcat/Km = 59,400 M-1s-1) at a rate 60X faster than hydrolysis by MMP-1 (interstitial collagenase). NFF-2 shows little discrimination between MMP-3, MMP-2 (gelatinase-A; kcat/Km = 54,000 M-1s-1), and MMP-9 (gelatinase-B; kcat/Km = 55,300 M-1s-1).
          FormLyophilized yellow solid
          FormulationSupplied as a trifluoroacetate salt.
          Chemical formulaC₇₉H₁₀₅N₁₉O₁₉S
          Peptide SequenceMCA-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(DNP)-NH₂ (DNP = 2,4-dinitrophenyl; Nva = L-norvaline)
          Purity≥98% by HPLC
          SolubilityDMSO (5 mg/ml)
          Storage -20°C
          Protect from moisture
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Toxicity Standard Handling
          ReferencesNagase, H., et al. 1994. J. Biol. Chem. 269, 20952.