SCC048 N27 Rat Dopaminergic Neural Cell Line

SCC048
≥1X10⁶ cells/vial  
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      CULT, Cult
      Description
      Catalogue NumberSCC048
      DescriptionN27 Rat Dopaminergic Neural Cell Line
      OverviewThe N27 rat dopaminergic neuron cell line was harvested from E12 rat mesencephalic tissue and was transfected with SV40 to immortalize the cell line. The N27 cell line, when injected into the striata of 6-hydroxydopamine-lesioned rats (an animal model of PD) caused a time-dependent improvement in neurological deficits. This immortalized cell line has been carefully characterized in studies of dopamine biosynthesis, neurotoxicity and used as a dopaminergic neuron model for in vitro and in vivo studies.
      Alternate Names
      • Dopamine Neural Cells
      • 1RB3AN27 Cell Line
      Background InformationParkinson’s disease is a neurodegenerative disorder of the central nervous system that affects more than 6 million people worldwide. The motor symptoms of Parkinson's disease result from the death of dopamine generating cells in the substantia nigra, a region of the midbrain. Numerous efforts have been made to slow the loss or replace these dopamine producing neurons in vivo.
      References
      Product Information
      Applications
      ApplicationThe N27 rat dopaminergic neural cell line has been used as a dopaminergic neuron model for in vitro and in vivo studies.
      Key Applications
      • Cell Culture
      • Stem Cell Culture
      Application NotesThis product is intended for sale and sold solely for internal non-commercial research use per the terms of the “Restricted Use Agreement” as detailed in the product documentation. For information regarding any other uses, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Neural Stem Cells
      • Neural Lineage Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X106 viable cells.
      • Cells are negative for mycoplasma contamination.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsN27 cells should be stored in liquid nitrogen. The cells can be passage for at least 10 passages without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      N27 Rat Dopaminergic Neural Cell Line Certificates of Analysis

      TitleLot Number
      N27 Rat Dopaminergic Neural Cell Line - 30772293077229

      References

      Reference overviewPub Med ID
      Protein targets of acrylamide adduct formation in cultured rat dopaminergic cells.
      Martyniuk, Christopher J, et al.
      Toxicol. Lett., 219: 279-87 (2013)  2013

      Show Abstract
      23566896 23566896
      Nicotinamide N-methyltransferase expression in SH-SY5Y neuroblastoma and N27 mesencephalic neurones induces changes in cell morphology via ephrin-B2 and Akt signalling.
      Thomas, M G, et al.
      Cell Death Dis, 4: e669 (2013)  2013

      Show Abstract
      23764850 23764850
      PKCδ mediates paraquat-induced Nox1 expression in dopaminergic neurons.
      Cristóvão, Ana Clara, et al.
      Biochem. Biophys. Res. Commun., 437: 380-5 (2013)  2013

      Show Abstract
      23827392 23827392
      Alterations in bioenergetic function induced by Parkinson's disease mimetic compounds: lack of correlation with superoxide generation.
      Dranka, Brian P, et al.
      J. Neurochem., 122: 941-51 (2012)  2012

      Show Abstract
      22708893 22708893
      The protective effect of astrocyte-derived 14,15-epoxyeicosatrienoic acid on hydrogen peroxide-induced cell injury in astrocyte-dopaminergic neuronal cell line co-culture.
      Terashvili, M, et al.
      Neuroscience, 223: 68-76 (2012)  2012

      Show Abstract
      22863680 22863680
      Thioredoxin reductase deficiency potentiates oxidative stress, mitochondrial dysfunction and cell death in dopaminergic cells.
      Lopert, Pamela, et al.
      PLoS ONE, 7: e50683 (2012)  2012

      Show Abstract
      23226354 23226354
      Improvement of neurological deficits in 6-hydroxydopamine-lesioned rats after transplantation with allogeneic simian virus 40 large tumor antigen gene-induced immortalized dopamine cells.
      Clarkson, E D, et al.
      Proc. Natl. Acad. Sci. U.S.A., 95: 1265-70 (1998)  1998

      Show Abstract
      9448320 9448320
      Characterization and transplantation of two neuronal cell lines with dopaminergic properties.
      Adams, F S, et al.
      Neurochem. Res., 21: 619-27 (1996)  1996

      Show Abstract
      8726972 8726972
      Establishment and characterization of immortalized clonal cell lines from fetal rat mesencephalic tissue.
      Prasad, K N, et al.
      In Vitro Cell. Dev. Biol. Anim., 30A: 596-603 (1994)  1994

      Show Abstract
      7820310 7820310

      Data Sheet

      Title
      N27 Rat Dopaminergic Neural Cell Line