|What is the blue separation paper between my filters?
||It is a parchmentalized paper specially formulated for Millipore Corporation.
|How do I tell the membrane from the separator?
||The blue paper is the separator.
|Which membrane should I use to filter my solution?
||For general filtration use MCE. When you are looking for the lowest protein binding membrane use Durapore. For speed, and when filtering serum solutions use Millipore Express.
|Which side of the membrane should I use, the shiny or dull side?
||Most researchers may not even notice that there is a "sidedness" to filters, and, for most applications, orientation will not affect filter performance. However, membranes do have a slightly asymmetric pore structure: the shiny side of the membrane is the "tighter" side. In some applications, you can take advantage of this difference by selecting a specific filter orientation. ultrafiltration membranes should always be used shiny side up, regardless of application for drop dialysis ( a buffer exchange technique in which a few drops of DNA or protein are placed in a 0.05 or 0.025 um filter and floated on a buffer solution), apply the sample to the shiny side of the filter and float the filter dull side to the buffer. This measure will enhance buffer exchange and discourage sample loss. The Millipore Express and Express Plus membranees are also sided - these membranes should be used shiny side facing down.
|What is the difference between pore size and pore size distribution?
||Whereas pore size is a measure of the diameter of the largest pore, pore size distribution is a measure of the range of pore sizes. The range of pore sizes can be normally distributed, and the spread can be quite narrow (e.g. the ratio of largest to smallest may be less than 2). On the other hand, pore size distribution can be very heterogeneous. In the case of large spreads and heterogeneity, the pore size will be far less predictive of flow rate (either filtration or capillary) than it will be for a membrane with a narrow pore size distribution. It is important to note that the pore size corresponding to the bubble point is not at the middle of the distribution, but is the largest pore.
|Is there a sidedness to the Express PES membrane?
||Yes, this membrane should be oriented shiny side down.
|Can Millipore filters remove mycoplasma from my solution?
||Mycoplasma can be removed from solutions by using a 0.1 um pore size filter. For mycoplasma reduction the 0.1 um Durapore filter can be used. Its Millipore code is VV. A 0.1 um Express membrane can also be used for removal of mycoplasma. Its membrane code is VP. These membranes are available in a number of fabricated fiter devices. For help determining which device would be best for your solution, call Millipore Technical Services.
|Is the Express membrane surfactant free?
||Yes, the Express membrane is surfactant free. It also has a very low extractables specification - less then 1.5% by weight.