SCM040 MilliTrace Rat Neural Stem Cell Expansion Medium

SCM040
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      Cult
      Description
      Catalogue NumberSCM040
      Trade Name
      • Chemicon
      DescriptionMilliTrace Rat Neural Stem Cell Expansion Medium
      OverviewMilliTrace Rat Neural Stem Cell Expansion Medium is provided as a multi-component system that has been optimized for use with the MilliTrace line of GFP reporter rat neural stem cells. The Neural Stem Cell Basal Medium is a defined serum-free medium that helps support the growth and in vitro differentiation of neural stem cells derived from rodents. Basic Fibroblast Growth Factor (FGF-2, bFGF) is provided separately, which when added to the Neural Stem Cell Basal Medium (SCM003) allows for the growth and proliferation of rat neural stem cells. Withdrawal of FGF-2 from the Neural Stem Cell Basal Medium will result in the spontaneous differentiation of rat neural stem cells. Puromycin Solution is provided separately to help maintain the expression of the GFP labeled transgene.
      Materials Required but Not Delivered· MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cell Kit (Millipore Catalog No. SCR080)
      · Neural Stem Cell Characterization Kit (Millipore Catalog No. SCR019)
      · Poly-L-ornithine (Sigma Catalog No. P3655)
      · Laminin (Millipore Catalog No. CC095)
      · Accutase™ (Millipore Catalog No. SCR005)
      References
      Product Information
      Components
      • Neural Stem Cell Basal Medium, 500 mL
      • Basic FGF, 10 µg
      • Puromycin Solution, 100 µL
      Applications
      ApplicationMilliTrace Rat Neural Stem Cell Expansion Medium is provided as a multi-component system that has been optimized for use with the MilliTrace line of GFP reporter rat neural stem cells.
      Key Applications
      • Stem Cell Culture
      Biological Information
      Media FormLiquid
      Stem Cell Type
      • Neural Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions· Neural Stem Cell Basal Medium (Part No. SCM003) should be stored at -20°C until ready to use. Upon thawing, the basal medium should be stored at 2-8°C for up to one month.
      · Basic FGF (10 µg, lyophilized) should be reconstituted with 100 µL 5 mM Tris-HCL, pH 7.6 for a final concentration of 100 µg/mL. Dispense into aliquots to avoid repeated freeze-thaw. Store at -20°C for up to six months. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Multiple freeze/thaw cycles will result in significant loss of activity.
      · 5 mg/mL Puromycin Solution, 100 µL (Part No. CS201361) should be stored in working aliquots at -20°C for up to two years.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MilliTrace Rat Neural Stem Cell Expansion Medium SDS

      Title

      Safety Data Sheet (SDS) 

      MilliTrace Rat Neural Stem Cell Expansion Medium Certificates of Analysis

      TitleLot Number
      MILLITRACE RAT NEURAL STEM CELL EXPANSION MEDIUM - 21351082135108
      MILLITRACE RAT NEURAL STEM CELL EXPANSION MEDIUM - 21459442145944
      MILLITRACE RAT NEURAL STEM CELL EXPANSION MEDIUM - 19780291978029
      MILLITRACE RAT NEURAL STEM CELL EXPANSION MEDIUM - 21932992193299
      MILLITRACE RAT NEURAL STEM CELL EXPANSION MEDIUM - 21960142196014
      MILLITRACE RAT NEURAL STEM CELL EXPANSION MEDIUM - 22433472243347
      MILLITRACE RAT NEURAL STEM CELL EXPANSION MEDIUM - NG1865584NG1865584
      MILLITRACE RAT NEURAL STEM CELL EXPANSION MEDIUM - NG1885812NG1885812
      MILLITRACE RAT NEURAL STEM CELL EXPANSION MEDIUM - NG1910734NG1910734
      MILLITRACE™ RAT NEURAL STEM CELL EXPANSION MEDIUM - 24927982492798

      Technical Info

      Title
      MilliTrace Constitutive GFP Reporter Neural Stem Cell Lines

      Data Sheet

      Title
      MILLITRACE™ RAT NEURAL STEM CELL EXPANSION MEDIUM

      Related Products & Applications

      Accessories

      Catalog Number Description  
      SCR080 MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cell Kit Show Pricing & Availability

      Categories

      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Stem Cell Culture Media