SCM041 MilliTrace Mouse Neural Stem Cell Expansion Medium

SCM041
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      Cult
      Description
      Catalogue NumberSCM041
      Trade Name
      • Chemicon
      DescriptionMilliTrace Mouse Neural Stem Cell Expansion Medium
      OverviewMilliTrace Mouse Neural Stem Cell Expansion Medium is provided as a multi-component system that has been optimized for use with the MilliTrace line of GFP reporter mouse neural stem cells. The Neural Stem Cell Basal Medium is a defined serum-free medium that helps support the growth and in vitro differentiation of neural stem cells derived from rodents. Basic Fibroblast Growth Factor (FGF-2, bFGF), Epidermal Growth Factor (EGF), and Heparin are provided separately, which when added to the Neural Stem Cell Basal Medium (SCM003) allow for the growth and proliferation of mouse neural stem cells. Withdrawal of the growth factors and heparin from the Neural Stem Cell Basal Medium will result in the spontaneous differentiation of mouse neural stem cells. Puromycin Solution is provided separately to help maintain the expression of the GFP labeled transgene.
      Materials Required but Not Delivered· MilliTrace Constitutive GFP Reporter Mouse Cortical Neural Stem Cell Kit (Millipore Catalog No. SCR081)
      · Neural Stem Cell Characterization Kit (Millipore Catalog No. SCR019)
      · Poly-L-ornithine (Sigma Catalog No. P3655)
      · Laminin (Millipore Catalog No. CC095)
      · Accutase™ Dissociation Solution (Millipore Catalog No. SCR005)
      References
      Product Information
      Components
      • Neural Stem Cell Basal Medium, 500 mL
      • Basic FGF, 10 µg
      • EGF Solution, 100 µL
      • Heparin Solution, 150 µL
      • Puromycin Solution, 100 µL
      Applications
      ApplicationMilliTrace Mouse Neural Stem Cell Expansion Medium is provided as a multi-component system that has been optimized for use with the MilliTrace line of GFP reporter mouse neural stem cells.
      Key Applications
      • Stem Cell Culture
      Biological Information
      Media FormLiquid
      Stem Cell Type
      • Neural Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions· Neural Stem Cell Basal Medium (Part No. SCM003) should be stored at -20°C until ready to use. Upon thawing, the basal medium should be stored at 2-8°C for up to one month.
      · Basic FGF (10 µg, lyophilized) should be reconstituted with 100 µL 5 mM Tris-HCL, pH 7.6 for a final concentration of 100 µg/mL. Dispense into aliquots to avoid repeated freeze-thaw. Store at -20°C for up to six months. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Multiple freeze/thaw cycles will result in significant loss of activity.
      · EGF and heparin solutions should be stored in working aliquots at -20°C for up to six months. The solutions can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Multiple freeze/thaw cycles will result in significant loss of activity.
      · 5 mg/mL Puromycin Solution, 100 µL (Part No. CS201361) should be stored in working aliquots at -20°C for up to two years.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MilliTrace Mouse Neural Stem Cell Expansion Medium Certificates of Analysis

      TitleLot Number
      MILLITRACE MOUSE NEURAL STEM CELL EXPANSION MEDIUM - 21282452128245
      MILLITRACE MOUSE NEURAL STEM CELL EXPANSION MEDIUM - 19636081963608
      MILLITRACE MOUSE NEURAL STEM CELL EXPANSION MEDIUM - 22626722262672
      MILLITRACE™ MOUSE NEURAL STEM CELL EXPANSION MEDIU2482242
      MILLITRACE™ MOUSE NEURAL STEM CELL EXPANSION MEDIUM -25042992504299
      MILLITRACE™ MOUSE NEURAL STEM CELL EXPANSION MEDIUM -25274882527488
      MILLITRACE™ MOUSE NEURAL STEM CELL EXPANSION MEDIUM - 24119042411904

      Technical Info

      Title
      MilliTrace Constitutive GFP Reporter Neural Stem Cell Lines

      Data Sheet

      Title
      MILLITRACE™ MOUSE NEURAL STEM CELL EXPANSION MEDIUM

      Related Products & Applications

      Accessories

      Catalog Number Description  
      SCR081 MilliTrace Constitutive GFP Reporter Mouse Cortical Neural Stem Cell Kit Show Pricing & Availability

      Categories

      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Stem Cell Culture Media