SCR080 MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cell Kit

SCR080
1 kit  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      Cult
      Description
      Catalogue NumberSCR080
      Trade Name
      • MilliTrace
      • Chemicon
      DescriptionMilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cell Kit
      OverviewMilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cell Kit provides ready-to use primary rat neural stem cells that are constitutively labeled with the humanized mulleri green fluorescent protein (hmGFP) along with expansion medium to help maintain expression of the transgene. hmGFP expression is driven off a constitutive promoter, chicken actin. These stable GFP transfected cells have been FACS sorted to over 95-100% purity.

      The constitutive GFP Reporter Adult Rat Hippocampal NSCs display the immunochemical staining properties of NSC; they are Nestin+Sox2+TuJ1-GFAP-. These cells are multipotent. Under defined neuronal differentiation conditions (Rodent Neuron Differentiation Kit, Millipore Cat. No. SCR035), the majority of cells are differentiated to βIII-tubulin positive neurons (GFP+βIII-tubulin+GFAP-). Under defined astrocytic differentiation conditions (Astrocyte Differentiation Medium, Millipore Cat. No. SCM010), majority of cells differentiate to GFAP-positive astrocytes (GFP+GFAP+βIII-tubulin-).
      References
      Product Information
      Components
      • MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cells, 1 x 106 viable cells
      • MilliTrace Rat Neural Stem Cell Expansion Medium (Cat. No. SCM040)
      Applications
      ApplicationMilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cell Kit provides ready-to use primary rat neural stem cells that are constitutively labeled with hmGFP along with expansion medium to help maintain expression of the transgene.
      Key Applications
      • Stem Cell Culture
      Biological Information
      Stem Cell Type
      • Neural Stem Cells
      Cell Line Type
      • Neural Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssurancePrimary constitutive GFP reporter rat neural stem cells have been validated for high level of constitutive expression of GFP, high level of expression of Nestin and Sox 2, and for their self-renewal and multi-lineage differentiation capacities. Cells display normal karyotype as assessed by chromosome spread and tested negative for mycoplasma.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • For Research Use Only. EMD Millipore does not recommend that a user derive clones from cells provided with this kit. However, should a user decide to do so, they may need a license to one or more third party patents and it is incumbent upon that user to determine whether and if so which third party patents need to be licensed.
      Storage and Shipping Information
      Storage Conditions· MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cells (Part No. SCC080) should be stored in Liquid Nitrogen. We recommend that the cells be used within ten passages.
      · Neural Stem Cell Basal Medium (Part No. SCM003) should be stored at -20°C until expiration date on the label. Upon thawing the basal medium should be stored at 2-8°C and given a 1-month expiration dating.
      · 5 mg/mL Puromycin Solution (Part No. CS201361) should be stored in working aliquots at -20°C for up to 2 years.
      · Lyophilized FGF-2 (Part No. GF003-10UG) should be reconstituted with 100 µL 5 mM Tris-HCL, pH 7.6 for a final concentration of 100 µg/mL. Dispense into aliquots to avoid repeated freeze-thaw. Store at -20°C for up to six months. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Multiple freeze/thaw cycles will result in significant loss of activity.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications

      Related Products & Applications

      Accessories

      Catalog Number Description  
      SCM040 MilliTrace Rat Neural Stem Cell Expansion Medium Show Pricing & Availability
      SCR019 Neural Stem Cell Marker Characterization Kit Show Pricing & Availability
      SCR035 Rodent Neuron Differentiation Kit Show Pricing & Availability

      Related Products By: Brand Facete

      Categories

      Life Science Research > Cell Culture and Systems > Cell Lines > Stem Cell Lines