475815 | Microcystin-LR, Microcystis aeruginosa - CAS 101043-37-2 - Calbiochem

475815
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical FormulaCAS #
      C₄₉H₇₄N₁₀O₁₂ 101043-37-2

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      475815-500UG
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          Alu drum 500 μg
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          Description
          OverviewCyclic heptapeptide toxin isolated from the freshwater cyanobacteria Microcystis aeruginosa. Potent inhibitor of protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A). Unlike Okadaic Acid (Cat. No. 495604), microcystin-LR is effective on both PP1 (IC50 = 1.7 nM) and PP2A (IC50 = 40 pM). Reacts with phosphatase in a two-step mechanism involving rapid binding and inactivation of the catalytic subunit followed by slower covalent interactions. Has no effect on protein kinases, making it useful for reducing the effect of contaminating phosphatase activities in protein kinase assays. A 2.5 mM (250 µg/100 µl) solution of Microcystin-LR, Microcystis aeruginosa (Cat. No. 475821) in DMSO is also available.
          Catalogue Number475815
          Brand Family Calbiochem®
          SynonymsMCYST-LR
          References
          ReferencesCraig, M., et al. 1996. Biochem. Cell Biol. 74, 569.
          Nishiwaki, R., et al. 1994. Cancer Lett. 83, 283.
          Amick, G.D., et al. 1992. Biochem. J. 287, 1019.
          Davidson, H.W., et al. 1992. J. Cell Biol. 116, 1343.
          Nishiwaki-Matsushima, R., et al. 1992. J. Cancer Res. Clin. Oncol. 118, 420.
          Cohen, P., et al. 1990. Trends Biochem. Sci. 15, 98.
          MacKintosh, C., et al. 1990. FEBS Lett. 264, 187.
          Cohen, P., et al. 1989. FEBS Lett. 250, 596.
          Carmichael, W.W. 1988. in Handbook of Natural Toxins 3: Marine Toxins and Venoms (Tu, A.T., Ed.) p. 121, Decker, New York.
          Product Information
          CAS number101043-37-2
          ATP CompetitiveN
          DeclarationNot available for sale outside of the United States.
          FormLyophilized pale-yellow solid
          Hill FormulaC₄₉H₇₄N₁₀O₁₂
          Chemical formulaC₄₉H₇₄N₁₀O₁₂
          ReversibleN
          Structure formula ImageStructure formula Image
          Applications
          ApplicationMicrocystin-LR, Microcystis aeruginosa - CAS 101043-37-2, is a potent inhibitor of protein phosphatase 1 (IC50 = 1.7 nM) and protein phosphatase 2A (IC50 = 40 pM).
          Biological Information
          Primary TargetPP1
          Primary Target IC<sub>50</sub>1.7 nM and 40 pM against PP1 and PP2A, respectively
          Purity≥95% by HPLC
          Physicochemical Information
          Cell permeableN
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSGT2810000
          Safety Information
          R PhraseR: 26/27/28-36/37/38-43

          Very toxic by inhalation, in contact with skin and if swallowed.
          Irritating to eyes, respiratory system and skin.
          May cause sensitization by skin contact.
          S PhraseS: 26-36/37/39-45

          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing, gloves and eye/face protection.
          In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Highly Toxic
          Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Unstable at > pH 7.7.
          End use certificateY
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          475815

          References

          Reference overview
          Craig, M., et al. 1996. Biochem. Cell Biol. 74, 569.
          Nishiwaki, R., et al. 1994. Cancer Lett. 83, 283.
          Amick, G.D., et al. 1992. Biochem. J. 287, 1019.
          Davidson, H.W., et al. 1992. J. Cell Biol. 116, 1343.
          Nishiwaki-Matsushima, R., et al. 1992. J. Cancer Res. Clin. Oncol. 118, 420.
          Cohen, P., et al. 1990. Trends Biochem. Sci. 15, 98.
          MacKintosh, C., et al. 1990. FEBS Lett. 264, 187.
          Cohen, P., et al. 1989. FEBS Lett. 250, 596.
          Carmichael, W.W. 1988. in Handbook of Natural Toxins 3: Marine Toxins and Venoms (Tu, A.T., Ed.) p. 121, Decker, New York.

          Brochure

          Title
          Protein Phosphatases Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision08-August-2008 RFH
          SynonymsMCYST-LR
          DescriptionCyclic heptapeptide toxin isolated from the freshwater cyanobacteria, Microcystis aeruginosa. Potent inhibitor of protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A). Unlike Okadaic Acid (Cat. No. 495604), microcystin-LR is effective on both PP1 (IC50 = 1.7 nM) and PP2A (IC50 = 40 pM). Has no effect on protein kinases, making it useful for reducing contaminating phosphatase activities in protein kinase assays.
          FormLyophilized pale-yellow solid
          CAS number101043-37-2
          RTECSGT2810000
          Chemical formulaC₄₉H₇₄N₁₀O₁₂
          Structure formulaStructure formula
          Purity≥95% by HPLC
          SolubilityDMSO (2.5 mg/ml) or Methanol (2 mg/ml). Further dilute with aqueous buffer just prior to use.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Unstable at > pH 7.7.
          Toxicity Highly Toxic
          ReferencesCraig, M., et al. 1996. Biochem. Cell Biol. 74, 569.
          Nishiwaki, R., et al. 1994. Cancer Lett. 83, 283.
          Amick, G.D., et al. 1992. Biochem. J. 287, 1019.
          Davidson, H.W., et al. 1992. J. Cell Biol. 116, 1343.
          Nishiwaki-Matsushima, R., et al. 1992. J. Cancer Res. Clin. Oncol. 118, 420.
          Cohen, P., et al. 1990. Trends Biochem. Sci. 15, 98.
          MacKintosh, C., et al. 1990. FEBS Lett. 264, 187.
          Cohen, P., et al. 1989. FEBS Lett. 250, 596.
          Carmichael, W.W. 1988. in Handbook of Natural Toxins 3: Marine Toxins and Venoms (Tu, A.T., Ed.) p. 121, Decker, New York.