17-20000 | Magna ChIP™ G Tissue Kit

17-20000
22 ChIP assays from tissue samples.  
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      Overview

      Replacement Information
      Description
      Catalogue Number17-20000
      Trade Name
      • Magna ChIP
      DescriptionMagna ChIP™ G Tissue Kit
      OverviewChromatin Immunoprecipitation (ChIP) is an important technique allowing the researcher to analyze in vivo interactions of proteins with genomic DNA. Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists. One can measure different proteins localized to a specific region of the genome, or the genome wide distribution of a specific protein. Another powerful application of this technique is to analyze changes in histone modifications that correlate with processes like transcription, mitosis or DNA repair.

      Historically, ChIP protocols have been geared towards homogenous populations of cell culture. In contrast, tissue samples are more complex and the cells are heterogeneous thus making ChIP analysis less amenable. The Magna ChIP™ G Tissue Kit provides the tools necessary to obtain repeatable, reliable, and site specific tissue biopsies. Microdissected functionally related populations of cells within a heterogeneous tissue can thus be analyzed with ease, precision and certainty.
      Materials Required but Not DeliveredCells, stimulated or treated as desired
      Antibody for chromatin immunoprecipitation
      37% Formaldehyde
      Taq DNA polymerase and dNTPs
      Master Mix for qPCR
      DNase and RNase-free sterile H2O
      Quant-iT™ dsDNA HS Assay Kit, (Life Technologies catalog #: Q32851)
      O.C.T. – Optimal Cutting Temperature compound
      RNase A
      Cryostat-microtome
      Cryomold
      Magna Grip™ Rack 8 well (Cat.#20-400) or similar magnetic rack
      Vortex mixer
      Rotating wheel/platform
      Microfuge
      Sonicator
      Thermomixer or Hybridization Oven
      Variable temperature water bath or incubator
      Variable volume (5-1000 l) pipettors + tips
      Microfuge & PCR tubes
      Thermal cycler
      Filter-tip pipette tips
      References
      Product Information
      Components
      • Magnetic Protein G beads
      • ChIP Dilution Buffer
      • Low Salt Wash Buffer
      • High Salt Wash Buffer
      • LiCl Wash Buffer
      • TE Buffer
      • Tissue Lysis Buffer
      • 10X Tissue Stabililizing Solution
      • ChIP Elution buffer (w/o Proteinase K)
      • 10X Glycine
      • 10X PBS
      • 1mm Micro-dissection punch
      • Spin Filters
      • Collection Tubes
      • Bind Reagent A
      • Wash Reagent B
      • Elution Reagent C
      • Protease Inhibitor Cocktail II
      • Proteinase K
      PresentationTwo modules containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions from tissue samples.
      Applications
      ApplicationThe Magna ChIP G Tissue Kit provides the tools necessary to obtain repeatable, reliable & site specific tissue biopsies.
      Key Applications
      • Sample Prep
      Biological Information
      Analytes Available
      • Protein G
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsUpon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when used and stored as directed.
      Packaging Information
      Material Size22 ChIP assays from tissue samples.
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      NADPH oxidase 4 and endothelial nitric oxide synthase contribute to endothelial dysfunction mediated by histone methylations in metabolic memory.
      Liao, Y; Gou, L; Chen, L; Zhong, X; Zhang, D; Zhu, H; Lu, X; Zeng, T; Deng, X; Li, Y
      Free Radic Biol Med  383-394  2018

      Show Abstract
      29269309 29269309
      Genome-wide mapping of estrogen receptor α binding sites by ChIP-seq to identify genes related to sexual maturity in hens.
      Guo, M; Li, Y; Chen, Y; Guo, X; Yuan, Z; Jiang, Y
      Gene  32-42  2018

      Show Abstract
      29128632 29128632
      Adult exposure to bisphenol A in rare minnow Gobiocypris rarus reduces sperm quality with disruption of testicular aquaporins.
      Zhang, Y; Guan, Y; Zhang, T; Yuan, C; Liu, Y; Wang, Z
      Chemosphere  365-375  2018

      Show Abstract
      29149713 29149713
      miR-126-5p promotes retinal endothelial cell survival through SetD5 regulation in neurons.
      Villain, G; Poissonnier, L; Noueihed, B; Bonfils, G; Rivera, JC; Chemtob, S; Soncin, F; Mattot, V
      Development  2018

      Show Abstract
      29180574 29180574
      Effects of adolescent social stress and antidepressant treatment on cognitive inflexibility and Bdnf epigenetic modifications in the mPFC of adult mice.
      Xu, H; Wang, J; Zhang, K; Zhao, M; Ellenbroek, B; Shao, F; Wang, W
      Psychoneuroendocrinology  92-101  2018

      Show Abstract
      29195162 29195162
      Bisphenol A regulates rare minnow testicular vitellogenin expression via reducing its promoter Er recruitment.
      Zhang, Y; Guan, Y; Fan, X; Wu, L; Wang, Z
      Ecotoxicol Environ Saf  423-429  2018

      Show Abstract
      28888792 28888792
      MeCP2 Deficiency Leads to Loss of Glial Kir4.1.
      Kahanovitch, U; Cuddapah, VA; Pacheco, NL; Holt, LM; Mulkey, DK; Percy, AK; Olsen, ML
      eNeuro  2018

      Show Abstract
      29464197 29464197
      Histone deacetylase inhibitors reverse age-related increases in side effects of haloperidol in mice.
      Montalvo-Ortiz, JL; Fisher, DW; Rodríguez, G; Fang, D; Csernansky, JG; Dong, H
      Psychopharmacology (Berl)  2385-2398  2017

      Show Abstract
      28421257 28421257
      Myc suppresses tumor invasion and cell migration by inhibiting JNK signaling.
      Ma, X; Huang, J; Tian, Y; Chen, Y; Yang, Y; Zhang, X; Zhang, F; Xue, L
      Oncogene  3159-3167  2017

      Show Abstract
      28068320 28068320
      A Wnt/Notch/Pax7 signaling network supports tissue integrity in tongue development.
      Zhu, XJ; Yuan, X; Wang, M; Fang, Y; Liu, Y; Zhang, X; Yang, X; Li, Y; Li, J; Li, F; Dai, ZM; Qiu, M; Zhang, Z; Zhang, Z
      J Biol Chem  9409-9419  2017

      Show Abstract
      28438836 28438836

      User Guides

      Title
      Magna ChIP¿ G Tissue Kit