17-610 | Magna ChIP™ A - Chromatin Immunoprecipitation Kit

17-610
22 assays  Kit capacity: 22 chromatin immunoprecipitation assays
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      Overview

      Replacement Information

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      For target-specific spike-in controls that make ChIP experiments more quantitative and accurate, Click on the Related Product & Applications tab above.
      Description
      Catalogue Number17-610
      Trade Name
      • Magna ChIP
      DescriptionMagna ChIP™ A - Chromatin Immunoprecipitation Kit
      OverviewChromatin Immunoprecipitation (ChIP) is an important technique allowing the researcher to analyze in vivo interactions of proteins with genomic DNA. Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists. One can measure different proteins localized to a specific region of the genome, or the genome wide distribution of a specific protein. Another powerful application of this technique is to analyze changes in histone modifications that correlate with processes like transcription, mitosis or DNA repair.

      Features & Benefits:
      Faster: Magnetic protein A beads allow for the entire ChIP protocol to be done in as little as a day! All reagents to process your samples are included - you don't have to spend valuable time making them.
      Easier: Spin columns make DNA purification easier and more reliable - no more messy phenol-chloroform extractions.
      Alternate Names
      • Magnetic ChIP Kit
      Materials Required but Not DeliveredMagna Grip™ Rack 8 well (Cat.# 20-400) (Now Available!) or similar magnetic rack.
      Background InformationChromatin Immunoprecipitation (ChIP) is a powerful technique for mapping the in vivo distribution of proteins associated with chromosomal DNA. These proteins can be histone subunits and post-translational modifications or other chromatin associated proteins such as transcription factors, chromatin regulators, etc. Additionally, ChIP can be used to identify regions of the genome associated with these proteins, or conversely, to identify proteins associated with a particular region of the genome. ChIP methodology often involves protein-DNA and protein-protein cross-linking, fragmentation of the cross-linked chromatin, and subsequent immunoprecipitation of chromatin with an antibody specific to a target protein. The DNA fragments isolated in complex with the target protein can be identified by a variety of methods including PCR, DNA microarray and DNA sequencing. Standard or quantitative PCR can be performed to verify whether a particular DNA sequence (the gene or region of the genome) is associated with the protein of interest. The combination of ChIP and promoter or genomic tiling microarrays (ChIP-chip) allows genome-wide identification of DNA-binding sites for chromatin-associated proteins with precise resolution. Alternatively, high-throughput sequencing of libraries constructed from immunoprecipitated chromosomal DNA (ChIP-Seq) is a powerful alternative to ChIP-chip in mapping the protein-DNA interactions across mammalian genomes.
      References
      Product Information
      Components
      • Magnetic Protein A Beads
      • ChIP Dilution Buffer
      • Low Salt Wash Buffer
      • High Salt Wash Buffer
      • LiCl Wash Buffer
      • TE Buffer
      • Cell Lysis Buffer
      • Nuclear Lysis Buffer
      • ChIP Elution Buffer (w/o Proteinase K)
      • 10X Glycine
      • 10X PBS
      • Protease Inhibitor Cocktail II
      • Proteinase K
      • Spin Filters
      • Collection Tubes
      • Bind Reagent A
      • Wash Reagent B
      • Elution Reagent C
      PresentationTwo boxes containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions. Supplied buffers are sufficient to generate chromatin from up to five 15 cm plates of cultured cells, each plate providing up to 10 chromatin preparations (varies with cell and assay type).
      Applications
      ApplicationSingle day chromatin immunoprecipitation (ChIP) kit containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using magnetic A beads.
      Biological Information
      Analytes Available
      • Protein A
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsUpon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 1 year from date of shipment when stored as directed.
      Packaging Information
      Material Size22 assays
      Material PackageKit capacity: 22 chromatin immunoprecipitation assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Magna ChIP A or EZ-Magna ChIP A Chromatin Immunoprecipitation Kits - 1982642 1982642
      Magna ChIP A or EZ-Magna ChIP A Chromatin Immunoprecipitation Kits - 2014533 2014533
      Magna ChIP A or EZ-Magna ChIP A Chromatin Immunoprecipitation Kits - 2032316 2032316
      Magna ChIP A or EZ-Magna ChIP A Chromatin Immunoprecipitation Kits - 2045692 2045692
      Magna ChIP A or EZ-Magna ChIP A Chromatin Immunoprecipitation Kits - 2089008 2089008
      Magna ChIP A or EZ-Magna ChIP A Chromatin Immunoprecipitation Kits - 2137004 2137004
      Magna ChIP A or EZ-Magna ChIP A Chromatin Immunoprecipitation Kits - 2153036 2153036
      Magna ChIP A or EZ-Magna ChIP A Chromatin Immunoprecipitation Kits - 2189711 2189711
      Magna ChIP A or EZ-Magna ChIP A Chromatin Immunoprecipitation Kits - 2197231 2197231
      Magna ChIP A or EZ-Magna ChIP A Chromatin Immunoprecipitation Kits - 2266497 2266497

      References

      Reference overviewPub Med ID
      ANKRD11 associated with intellectual disability and autism regulates dendrite differentiation via the BDNF/TrkB signaling pathway.
      Ka, M; Kim, WY
      Neurobiol Dis  111  138-152  2018

      Show Abstract
      29274743 29274743
      Atrx inactivation drives disease-defining phenotypes in glioma cells of origin through global epigenomic remodeling.
      Danussi, C; Bose, P; Parthasarathy, PT; Silberman, PC; Van Arnam, JS; Vitucci, M; Tang, OY; Heguy, A; Wang, Y; Chan, TA; Riggins, GJ; Sulman, EP; Lang, F; Creighton, CJ; Deneen, B; Miller, CR; Picketts, DJ; Kannan, K; Huse, JT
      Nat Commun  9  1057  2018

      Show Abstract
      29535300 29535300
      Discovery of a Glucocorticoid Receptor (GR) Activity Signature Using Selective GR Antagonism in ER-Negative Breast Cancer.
      West, DC; Kocherginsky, M; Tonsing-Carter, EY; Dolcen, DN; Hosfield, DJ; Lastra, RR; Sinnwell, JP; Thompson, KJ; Bowie, KR; Harkless, RV; Skor, MN; Pierce, CF; Styke, SC; Kim, CR; de Wet, L; Greene, GL; Boughey, JC; Goetz, MP; Kalari, KR; Wang, L; Fleming, GF; Győrffy, B; Conzen, SD
      Clin Cancer Res  0  2018

      Show Abstract
      29636357 29636357
      The long non-coding RNA 91H increases aggressive phenotype of breast cancer cells and up-regulates H19/IGF2 expression through epigenetic modifications.
      Vennin, C; Spruyt, N; Robin, YM; Chassat, T; Le Bourhis, X; Adriaenssens, E
      Cancer Lett  385  198-206  2017

      Show Abstract
      27780718 27780718
      Essential Roles for ARID1B in Dendritic Arborization and Spine Morphology of Developing Pyramidal Neurons.
      Ka, M; Chopra, DA; Dravid, SM; Kim, WY
      J Neurosci  36  2723-42  2016

      Show Abstract
      26937011 26937011
      Status of hepatic DNA methylome predetermines and modulates the severity of non-alcoholic fatty liver injury in mice.
      Tryndyak, VP; Han, T; Fuscoe, JC; Ross, SA; Beland, FA; Pogribny, IP
      BMC Genomics  17  298  2016

      Show Abstract
      27103143 27103143
      IL-6/STAT3 axis initiated CAFs via up-regulating TIMP-1 which was attenuated by acetylation of STAT3 induced by PCAF in HCC microenvironment.
      Zheng, X; Xu, M; Yao, B; Wang, C; Jia, Y; Liu, Q
      Cell Signal  28  1314-24  2016

      Show Abstract
      27297362 27297362
      FOXO1-mediated epigenetic modifications are involved in the insulin-mediated repression of hepatocyte aquaporin 9 expression.
      Qiu, LW; Gu, LY; Lü, L; Chen, XF; Li, CF; Mei, ZC
      Mol Med Rep  11  3064-8  2015

      Show Abstract
      25503267 25503267
      Genistein disrupts glucocorticoid receptor signaling in human uterine endometrial Ishikawa cells.
      Whirledge, S; Senbanjo, LT; Cidlowski, JA
      Environ Health Perspect  123  80-7  2015

      Show Abstract
      25136773 25136773
      Investigation of genes important in neurodevelopment disorders in adult human brain.
      Maussion, G; Diallo, AB; Gigek, CO; Chen, ES; Crapper, L; Théroux, JF; Chen, GG; Vasuta, C; Ernst, C
      Hum Genet  134  1037-53  2015

      Show Abstract
      26194112 26194112

      FAQ

      QuestionAnswer
      From where are the primer sequences derived for the kit?The primer sequences are based on the Human GAPDH promoter. The GenBank number is NT_009759.15, using nts:6497145-6498136.
      How many PCR reactions can be done with this kit?There are enough primers and PCR buffer for 4 reactions per IP assuming a 20 microliter volume and assuming the primers are at the recommended concentration as stated in the manual.

      User Guides

      Title
      Magna ChIP™ A

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      Categories

      Life Science Research > Kits & Assays > ChIP Kits > Kits and Assays
      Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > Chromatin Immunoprecipitation (ChIP) > ChIP Kits & Beads