475954 MRN-ATM Pathway Inhibitor, Mirin - CAS 299953-00-7 - Calbiochem

475954
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical FormulaCAS #
      C₁₀H₈N₂O₂S 299953-00-7

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      475954-10MG
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          Plastic ampoule 10 mg
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          Description
          OverviewA cell-permeable pyrimidinone compound that inhibits the exonuclease activity of Mre11, component of the MRN (Mre11-Rad50-Nbs1) complex, and prevents MRN-mediated activation of dimeric ATM (IC50 = 12 µM) in cell-free p53 Ser15 phosphorylation assays, while exhibiting little effect against MRN-independent ATM activation in the presence of high DSBs (DNA double strain breaks) or the activity of activated monomeric ATM. Mirin is shown to cause an increase of G2 population in exponentially growing TOSA4 cultures (~1.6-fold at 50 µM) and dramatically increase mitotic cell polulation (~10-fold at 25 µM) after irradiation of G2 phase U2OS cultures by preventing IR-induced G2/M checkpoint. Mirin exhibits no inhibitory activity against Rad50-associated adenylate kinase or Exonulease III.
          Catalogue Number475954
          Brand Family Calbiochem®
          Synonyms(Z)-5-(4-Hydroxybenzylidene)-2-imino-1,3-thiazolidin-4-one, Mirin
          References
          ReferencesGarner, K.M., et al. 2009. Nature Chem. Biol. 5, 129.
          Peterson, S., et al. 2009. Nature Chem. Biol. 5, 130.
          Dupre, A., et al. 2008. Nature Chem. Biol. 4, 119.
          Product Information
          CAS number299953-00-7
          FormReddish orange powder
          Hill FormulaC₁₀H₈N₂O₂S
          Chemical formulaC₁₀H₈N₂O₂S
          Structure formula ImageStructure formula Image
          Applications
          Biological Information
          Purity≥95% by HPLC
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          475954

          References

          Reference overview
          Garner, K.M., et al. 2009. Nature Chem. Biol. 5, 129.
          Peterson, S., et al. 2009. Nature Chem. Biol. 5, 130.
          Dupre, A., et al. 2008. Nature Chem. Biol. 4, 119.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision25-August-2009 RFH
          Synonyms(Z)-5-(4-Hydroxybenzylidene)-2-imino-1,3-thiazolidin-4-one, Mirin
          DescriptionA cell-permeable pyrimidinone compound that inhibits the exonuclease activity of Mre11, component of the MRN (Mre11-Rad50-Nbs1) complex, and prevents MRN-mediated activation of dimeric ATM (IC50 = 12 µM) in cell-free p53 Ser15 phosphorylation assays, while exhibiting little effect against MRN-independent ATM activation in the presence of high DSBs (DNA double strain breaks) or the activity of activated monomeric ATM. Mirin is shown to cause an increase of G2 population in exponentially growing TOSA4 cultures (~1.6-fold at 50 µM) and dramatically increase mitotic cell polulation (~10-fold at 25 µM) after irradiation of G2 phase U2OS cultures by preventing IR-induced G2/M checkpoint. Mirin exhibits no inhibitory activity against Rad50-associated adenylate kinase or Exonulease III.
          FormReddish orange powder
          Intert gas (Yes/No) Packaged under inert gas
          CAS number299953-00-7
          Chemical formulaC₁₀H₈N₂O₂S
          Structure formulaStructure formula
          Purity≥95% by HPLC
          SolubilityDMSO (10 mg/ml) or Ethanol (3 mg/ml)
          Storage +2°C to +8°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Toxicity Standard Handling
          ReferencesGarner, K.M., et al. 2009. Nature Chem. Biol. 5, 129.
          Peterson, S., et al. 2009. Nature Chem. Biol. 5, 130.
          Dupre, A., et al. 2008. Nature Chem. Biol. 4, 119.