475904 | MOWIOL® 4-88 Reagent

475904
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      475904-100GM
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      Available
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          Glass bottle 100 gm
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          Description
          OverviewUsed for mounting cells on slides for detection of cell-surface antigens by immunofluorescence. Useful as an anti-fading agent.
          Catalogue Number475904
          Brand Family Calbiochem®
          References
          ReferencesLongin, A., et al. 1993. J. Histochem. Cytochem. 41, 1833.
          Beug, H., et al. 1979. Cell 18, 375.
          Product Information
          CAS number9002-89-5
          FormYellow solid
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSTR8100000
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +15°C to +30°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 weeks at 4°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          475904

          References

          Reference overview
          Longin, A., et al. 1993. J. Histochem. Cytochem. 41, 1833.
          Beug, H., et al. 1979. Cell 18, 375.

          Citations

          Title
        • Marie Johansson, et al. (2007) Activation of endosomal dynein motors by stepwise assembly of Rab7–RILP–p150Glued, ORP1L, and the receptor ßlll spectrin. Journal of Cell Biology 176, 459-471.
        • Madhavi P. Maddugoda, et al. (2007) Myosin VI and vinculin cooperate during the morphogenesis of cadherin cell cell contacts in mammalian epithelial cells. Journal of Cell Biology 178, 529-540.
        • Aarti N. Desai, et al. (2006) Involvement of GRK3 and GRK2 in Down-regulation of the {alpha}2B-Adrenoceptor. Journal of Pharmacology and Experimental Therapeutics 317, 1027-1035.
        • Ganna Panasyuk, et al. (2006) Nuclear export of S6K1 II is regulated by protein kinase CK2 phosphorylation at ser 17. Journal of Biological Chemistry 281, 31188-31201.
        • Maria V. Baez and Graciela L. Boccaccio. (2005) Mammalian SMAUG is a translational repressor that forms cytoplasmic foci similar to stress granules. Journal of Biological Chemistry 280, 43131-43140.
        • Micah M. Bhatti and William J. Sullivan, Jr.. (2005) Histone acetylase GCN5 enters the nucleus via importin- in protozoan parasite Toxoplasma gondii. Journal of Biological Chemistry 280, 5902-5908.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision11-August-2008 RFH
          DescriptionUsed for mounting cells on slides for detection of cell-surface antigens by immunofluorescence. Useful as an anti-fading agent.
          FormYellow solid
          CAS number9002-89-5
          RTECSTR8100000
          SolubilityAdd 2.4 g of MOWIOL® 4-88 to 6 g of glycerol and stir to mix. Add 6 ml of water and stir for several hours at room temperature. Add 12 ml of 0.2 M Tris (pH 8.5) and heat to 50°C for 10 min. with occasional mixing. After the MOWIOL® 4-88 dissolves, clarify the solution by centrifugation at 5000 x g for 15 min.
          Storage +15°C to +30°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 weeks at 4°C.
          Toxicity Standard Handling
          ReferencesLongin, A., et al. 1993. J. Histochem. Cytochem. 41, 1833.
          Beug, H., et al. 1979. Cell 18, 375.
          Citation
        • Marie Johansson, et al. (2007) Activation of endosomal dynein motors by stepwise assembly of Rab7–RILP–p150Glued, ORP1L, and the receptor ßlll spectrin. Journal of Cell Biology 176, 459-471.
        • Madhavi P. Maddugoda, et al. (2007) Myosin VI and vinculin cooperate during the morphogenesis of cadherin cell cell contacts in mammalian epithelial cells. Journal of Cell Biology 178, 529-540.
        • Aarti N. Desai, et al. (2006) Involvement of GRK3 and GRK2 in Down-regulation of the {alpha}2B-Adrenoceptor. Journal of Pharmacology and Experimental Therapeutics 317, 1027-1035.
        • Ganna Panasyuk, et al. (2006) Nuclear export of S6K1 II is regulated by protein kinase CK2 phosphorylation at ser 17. Journal of Biological Chemistry 281, 31188-31201.
        • Maria V. Baez and Graciela L. Boccaccio. (2005) Mammalian SMAUG is a translational repressor that forms cytoplasmic foci similar to stress granules. Journal of Biological Chemistry 280, 43131-43140.
        • Micah M. Bhatti and William J. Sullivan, Jr.. (2005) Histone acetylase GCN5 enters the nucleus via importin- in protozoan parasite Toxoplasma gondii. Journal of Biological Chemistry 280, 5902-5908.