444282 | MMP/TACE Substrate, FS-6 - Calbiochem

444282
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      444282-1MG
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          Glass bottle 1 mg
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          Description
          OverviewThe widely used MMP substrate FS-1 (Cat. No. 03-32-5032) is modified by an N-terminal lysine elongation to produce a broad-spectrum MMP and TACE substrate that displays improved water solubility and substrate sensitivities. Exhibits 2- to 9-fold higher specificity constant (kcat/Km) towards MMP-1, -8, -13, -14 when compared with FS-1 and ~2-fold higher kcat/Km towards TACE when compared with TACE Substrate II and III (Cat. Nos. 616402 and 616403, respectively). It has been used successfully in detecting T98G glioma cell surface-associated MMP activity and is shown to be resistant to proteolysis by human leukocyte elastase, trypsin, and ADAM-10.
          Catalogue Number444282
          Brand Family Calbiochem®
          SynonymsMca-KPLG~L-Dpa-AR-NH₂
          References
          ReferencesNeumann, U., et al. 2004. Anal. Biochem. 328, 166.
          Product Information
          ATP CompetitiveN
          FormLyophilized
          FormulationSupplied as a trifluoroacetate salt.
          Hill FormulaC₅₅H₈₀N₁₆O₁₆
          Chemical formulaC₅₅H₈₀N₁₆O₁₆
          Hygroscopic Hygroscopic
          ReversibleN
          Applications
          Biological Information
          Primary TargetBroad-spectrum MMP and TACE substrate
          Purity≥97% by HPLC
          Physicochemical Information
          Cell permeableN
          Peptide SequenceMca-Lys-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH₂
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Hygroscopic Hygroscopic
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          444282

          References

          Reference overview
          Neumann, U., et al. 2004. Anal. Biochem. 328, 166.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision28-September-2007 RFH
          SynonymsMca-KPLG~L-Dpa-AR-NH₂
          DescriptionThe widely used MMP substrate FS-1 (Cat. No. 03-32-5032), modified by an N-terminal lysine elongation to produce a broad-spectrum MMP and TACE substrate that displays improved water solubility and sensitivity. Exhibits a 2- to 9-fold higher specificity constant (kcat/Km) towards MMP-1, -8, -13, -14 (Km (μM)= 27.5, 5.2, and 7.9, respectively) when compared with FS-1 and ~ 2-fold higher kcat/Km (778, 400 M-1 s-1) towards TACE when compared with TACE Substrate II and III (Cat. Nos. 616402 and 616403, respectively). It has been used successfully in detecting T98G glioma cell surface-associated MMP activity and is shown to be resistant to proteolysis by human leukocyte elastase, trypsin, and ADAM-10. Excitation max: 324 nm; Emission max: 400 nm.
          FormLyophilized
          FormulationSupplied as a trifluoroacetate salt.
          Intert gas (Yes/No) Packaged under inert gas
          Recommended reaction conditionsMMP: 100 mM Tris-HCL,100 mM Nacl, 10 mM CaCl2, 0-5% (w/v) BRIJ®35 detergent, 0.1% (w/v) PEG6000, pH7.5. TACE and ADAM10: (10 mM HEPES, pH 7.5).
          Chemical formulaC₅₅H₈₀N₁₆O₁₆
          Peptide SequenceMca-Lys-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH₂
          Purity≥97% by HPLC
          SolubilityH₂O (20 mg/ml)
          Storage +2°C to +8°C
          Hygroscopic
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
          Toxicity Standard Handling
          ReferencesNeumann, U., et al. 2004. Anal. Biochem. 328, 166.