444256 MMP Substrate III, Fluorogenic - Calbiochem

444256
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      444256-500UG
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          Plastic ampoule 500 μg
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          Description
          OverviewA specific fluorogenic resonance energy transfer (FRET) peptide substrate with an MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination. Useful for the detection of MMP activity [kcat/Km = 619,000 M-1s-1 for MMP-2, 206,000 M-1s-1 for MMP-9, 40,000 M-1s-1 for MMP-3, and 21,000 M-1s-1 for MMP-1; at 37°C, pH 7.6 ]. Exhibits a high degree of sensitivity that is not affected by optical disturbances in biological media. Also useful for MMP activity measurements in synovial fluid and culture medium.
          Catalogue Number444256
          Brand Family Calbiochem®
          SynonymsDABCYL-GABA-PQGL-E(EDANS)-AK-NH₂, TNO211
          References
          ReferencesBeekman, B., et al. 1996. FEBS Lett 390, 221.
          Product Information
          ATP CompetitiveN
          FormRed lyophilized solid
          FormulationSupplied as a trifluoroacetate salt.
          Hill FormulaC₆₃H₈₈N₁₆O₁₄S
          Chemical formulaC₆₃H₈₈N₁₆O₁₄S
          ReversibleN
          Applications
          Biological Information
          Primary TargetDetection of MMP activity
          Purity≥97% by HPLC
          Physicochemical Information
          Cell permeableN
          Emission max.
          Excitation max.
          Peptide SequenceDABCYL-GABA-Pro-Gln-Gly-Leu-Glu(EDANS)-Ala-Lys-NH₂
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          444256

          References

          Reference overview
          Beekman, B., et al. 1996. FEBS Lett 390, 221.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision07-August-2008 RFH
          SynonymsDABCYL-GABA-PQGL-E(EDANS)-AK-NH₂, TNO211
          DescriptionA fluorescence energy transfer (FRET) peptide substrate with MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination. Useful for the detection of MMP activity (kcat/Km = 619,000 M-1s-1 for MMP-2, 206,000 M-1s-1 for MMP-9, 40,000 M-1s-1 for MMP-3, 21,000 M-1s-1 for MMP-1; pH = 7.6 at 37°C). Exhibits a high degree of sensitivity, which is not affected by optical disturbances in biological media. Excitation max.: ~340 nm; emission max.: ~485 nm.
          FormRed lyophilized solid
          FormulationSupplied as a trifluoroacetate salt.
          Intert gas (Yes/No) Packaged under inert gas
          Chemical formulaC₆₃H₈₈N₁₆O₁₄S
          Peptide SequenceDABCYL-GABA-Pro-Gln-Gly-Leu-Glu(EDANS)-Ala-Lys-NH₂
          Purity≥97% by HPLC
          SolubilityDMSO and Tris buffered saline (pH 7.6)
          Storage -20°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
          Toxicity Standard Handling
          ReferencesBeekman, B., et al. 1996. FEBS Lett 390, 221.