454850 | MAP Kinase 2, Mouse, Recombinant, E. coli

454850
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      454850-2000U
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          Plastic ampoule 2000 u
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          Description
          OverviewRecombinant, mouse MAP Kinase 2 expressed in E. coli. A serine/threonine protein kinase that acts at the end of a protein kinase cascade linking signals from cell surface receptor tyrosine kinases to cytoplasmic and nuclear events. This protein was co-expressed with MEK and is phosphorylated and active.
          Catalogue Number454850
          Brand Family Calbiochem®
          SynonymsERK2, MAPK2, p42MAPK
          References
          ReferencesFukunaga, K., and Miyamoto, E. 1998. Mol. Neurobiol. 16, 79.
          Wu, J., et al. 1993. Mol. Cell. Biol. 13, 4539.
          Product Information
          Activity≥5000 units/ml
          Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein per min at 30°, pH 7.5.
          FormLiquid
          FormulationIn 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 100 µM Na₂-EDTA, 50% glycerol, 0.01% BRIJ® 35 detergent, pH 7.5.
          Applications
          Biological Information
          Purity≥90% by SDS-PAGE
          Physicochemical Information
          ContaminantsMEK, phosphatase, protease: none detected.
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          454850

          References

          Reference overview
          Fukunaga, K., and Miyamoto, E. 1998. Mol. Neurobiol. 16, 79.
          Wu, J., et al. 1993. Mol. Cell. Biol. 13, 4539.

          Brochure

          Title
          MAP Kinases Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision07-August-2008 RFH
          SynonymsERK2, MAPK2, p42MAPK
          DescriptionRecombinant, mouse MAP Kinase 2 expressed in E. coli. A serine/threonine protein kinase that acts at the end of a protein cascade linking signals from cell surface receptor tyrosine kinases to cytoplasmic and nuclear events. This protein was co-expressed with MEK and is phosphorylated and active.
          FormLiquid
          FormulationIn 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 100 µM Na₂-EDTA, 50% glycerol, 0.01% BRIJ® 35 detergent, pH 7.5.
          Recommended reaction conditions
          Note: This protocol is provided only as a general guide. Researchers should standardize this assay for their own specific needs and should consult published literature. Suggested Protocol to Assay for MAPK Activators 1. Combine the following on ice: 4.0 µl 250 mM HEPES, pH 7.5, 100 mM Mg(OAc)2, 500 µM ATP 2.0 µl MAP Kinase 1-29 µl purified MEK (cell lysates or fractions from chromatography) 2.0 µl [γ-32P]ATP (1.0 µCi/µl) add enough distilled H2O to produce a final volume of 40 µl. 2. Incubate at 30°C for 30 min. 3. Stop the reaction by adding 40 µl of 2X SDS-PAGE sample buffer. 4. The phosphorylated MAPK will show as a signal at ~45 kDa by autoradiography. Phosphorylating Protein Substrates with Phosphorylated MAPK 1. Combine the following on ice: 5.0 µl 500 mM Tris-HCl (pH 7.5), 62.5 mM β-glycerol phosphate, 50 mM MgCl2, 5 mM EGTA, 250 µM NaF, 5 mM DTT, and 2.5 mM sodium vanadate 2 µg experimental protein or Myelin Basic Protein Peptide Substrate (Cat. No. 475920) 1.0 µl phosphorylated MAPK 2.0 µl [γ-32P]ATP (1.25 mM,100 µCi/µmoll) add enough distilled H2O to produce a final volume of 25 µl. 2. Incubate at 30°C for 30 min. 3. Stop the reaction by spotting 20 µl of each reaction mixture onto a 1.5 cm2 piece of Whatman P81 paper. 4. Wash the squares 7X for 5 min with phosphoric acid (1% w/v). 5. Quantitate the 32P incorporation in a liquid scintillation counter.
          Purity≥90% by SDS-PAGE
          ContaminantsMEK, phosphatase, protease: none detected.
          Activity≥5000 units/ml
          Unit definitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein per min at 30°, pH 7.5.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesFukunaga, K., and Miyamoto, E. 1998. Mol. Neurobiol. 16, 79.
          Wu, J., et al. 1993. Mol. Cell. Biol. 13, 4539.