454854 MAP Kinase 2, His•Tag®, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: ERK2, MAPK2, p42MAPK

454854
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      454854-5UG
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      Stocked 
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          		 Plastic ampoule 5 μg
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          Description
          OverviewRecombinant, human MAP Kinase 2 fused to a His•Tag® sequence and expressed in E. coli. Highly active form produced by phosphorylation of the purified MAP Kinase in vitro with MEK1. Suitable for labeling MAP Kinase substrates.
          Catalogue Number454854
          Brand Family Calbiochem®
          SynonymsERK2, MAPK2, p42MAPK
          References
          ReferencesLew, J. 2003. Biochemistry 42, 849.
          Johnson, G.L., and Lapadat, R. 2002. Science 298, 1911.
          Koistinaho, M., and Koistinaho, J. 2002. Glia 40, 175.
          Product Information
          Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein (MBP) per min at 30°C.
          FormLiquid
          FormulationIn 150 mM NaCl, 50 mM Tris, 1 mM DTT, 50% glycerol, pH 8.5.
          Applications
          Biological Information
          Purity≥95% by SDS-PAGE
          Specific Activity≥5500 units/mg protein
          Physicochemical Information
          ContaminantsProteases: none detected
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          454854

          References

          Reference overview
          Lew, J. 2003. Biochemistry 42, 849.
          Johnson, G.L., and Lapadat, R. 2002. Science 298, 1911.
          Koistinaho, M., and Koistinaho, J. 2002. Glia 40, 175.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision07-August-2008 RFH
          SynonymsERK2, MAPK2, p42MAPK
          DescriptionRecombinant, human MAP Kinase 2 fused to a His•Tag® sequence and expressed in E. coli. Highly active form produced by phosphorylation of the purified MAP kinase in vitro with MEK1. Suitable for labeling MAP Kinase substrates.
          FormLiquid
          FormulationIn 150 mM NaCl, 50 mM Tris, 1 mM DTT, 50% glycerol, pH 8.5.
          Purity≥95% by SDS-PAGE
          ContaminantsProteases: none detected
          Specific activity≥5500 units/mg protein
          Unit definitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein (MBP) per min at 30°C.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesLew, J. 2003. Biochemistry 42, 849.
          Johnson, G.L., and Lapadat, R. 2002. Science 298, 1911.
          Koistinaho, M., and Koistinaho, J. 2002. Glia 40, 175.