Key Specifications Table
|Key Applications||Detection Methods|
|TFX, IF, ICC||Fluorescent|
|Description||LentiBrite™ Calreticulin-RFP-KDEL Lentiviral Biosensor|
|Overview||Read our application note in Nature Methods!
Learn more about the advantages of our LentiBrite Lentiviral Biosensors! Click Here
Biosensors can be used to detect the presence/absence of a particular protein as well as the subcellular location of that protein within the live state of a cell. Fluorescent tags are often desired as a means to visualize the protein of interest within a cell by either fluorescent microscopy or time-lapse video capture. Visualizing live cells without disruption allows researchers to observe cellular conditions in real time.
Lentiviral vector systems are a popular research tool used to introduce gene products into cells. Lentiviral transfection has advantages over non-viral methods such as chemical-based transfection including higher-efficiency transfection of dividing and non-dividing cells, long-term stable expression of the transgene, and low immunogenicity.
EMD Millipore is introducing LentiBrite™ Lentiviral Biosensors, a new suite of pre-packaged lentiviral particles encoding important and foundational proteins of autophagy, apoptosis, and cell structure for visualization under different cell/disease states in live cell and in vitro analysis.
EMD Millipore’s LentiBrite™ Calreticulin-RFP-KDEL lentiviral particles provide bright fluorescence and precise localization to enable live cell analysis of autophagy in difficult-to-transfect cell types.
|Background Information||Calreticulin is a resident protein of the lumen of the endoplasmic reticulum that plays important roles in protein folding and calcium homeostasis. In addition, cells induced to undergo apoptosis with selected agents allow passage of calreticulin from the ER to the cell surface, and the cell surface-exposed calreticulin promotes engulfment by phagocytic cells, a process termed immunogenic cell death. Expression of genetic fusions between calreticulin and fluorescent proteins has permitted analysis of the dynamics of chaperone-substrate interactions and immunogenic cell death.
EMD Millipore’s LentiBrite™ Calreticulin-RFP-KDEL lentiviral particles provide bright fluorescence and precise localization to enable live cell analysis of calreticulin dynamics in difficult-to-transfect cell types.
|Purification Method||PEG precipitation|
|Safety Information according to GHS|
|Product Usage Statements|
|Quality Assurance||Evaluated by transduction of HT-1080 cells and fluorescent imaging performed for assessment of target localization and transduction efficiency.|
|Material Size||1 vial (minimum of 3 x 10E8 IFU/mL)|