407631 | Interleukin-3, Mouse, Recombinant, E. coli

407631
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      407631-10UG
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          Plastic ampoule 10 μg
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          Description
          OverviewRecombinant, mouse interleukin-3 expressed in E. coli. Stimulates production and differentiation of macrophages, neutrophils, basophils, mast cells, and eosinophils.
          Catalogue Number407631
          Brand Family Calbiochem®
          SynonymsrmIL-3
          References
          ReferencesMuther, H., et al. 1994. Growth Factors 10, 17.
          Product Information
          FormLyophilized
          FormulationLyophilized from sterile-filtered (0.22 µm) buffer, carrier-free.
          Applications
          Biological Information
          Biological activityED₅₀ = 0.01-0.1 ng/ml as measured in a cell proliferation assay using NFS-60 cells.
          Purity≥95% by SDS-PAGE
          Physicochemical Information
          ContaminantsEndotoxin: ≤ 0.1 ng/µg IL-3
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          407631

          References

          Reference overview
          Muther, H., et al. 1994. Growth Factors 10, 17.

          Brochure

          Title
          Growth Factors and Cytokines Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision13-May-2008 RFH
          SynonymsrmIL-3
          DescriptionRecombinant, mouse interleukin-3 expressed in E. coli. IL-3 is a hemopoietic colony stimulating factor that interacts with very early multipotent hematopoietic progenitor cells. Stimulates production and differentiation of macrophages, neutrophils, basophils, mast cells, and eosinophils. IL-3 supports colony formation in the soft agar of bone marrow cells of granulocyte, macrophage, megakaryocyte, and erythrocyte lineages. Unlike human IL-3, the murine form supports the growth of mast cells. IL-3 activity is highly species-specific. Murine IL-3 does not show activity on human cells. Recombinant IL-3 produced in and purified from E. coli.
          FormLyophilized
          FormulationLyophilized from sterile-filtered (0.22 µm) buffer, carrier-free.
          Purity≥95% by SDS-PAGE
          ContaminantsEndotoxin: ≤ 0.1 ng/µg IL-3
          Biological activityED₅₀ = 0.01-0.1 ng/ml as measured in a cell proliferation assay using NFS-60 cells.
          SolubilitydH₂O. Reconstitute to a final concentration of 0.1-1 mg/ml. Further dilutions should be made in low-endotoxin medium or buffer containing FBS or tissue culture grade BSA.
          Storage ≤ -70°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.
          Toxicity Standard Handling
          Merck USA index14, 4995
          ReferencesMuther, H., et al. 1994. Growth Factors 10, 17.