ECM625 | In Vitro Angiogenesis Assay Kit

ECM625
1 kit  
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      ACT
      Description
      Catalogue NumberECM625
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionIn Vitro Angiogenesis Assay Kit
      OverviewAngiogenesis is the process of generating new capillary blood vessels. It is a fundamental component of a number of normal (reproduction and wound healing) and pathological processes (diabetic retinopathy, rheumatoid arthritis, tumor growth and metastasis).

      The CHEMICON In Vitro Angiogenesis Assay Kit provides a convenient system for evaluation of tube formation by endothelial cells in a convenient 96-well format. When cultured on ECMatrix™, a solid gel of basement proteins prepared from the Engelbreth Holm-Swarm (EHS) mouse tumor, these endothelial cells rapidly align and form hollow tube-like structures. Tube formation is a multi-step process involving cell adhesion, migration, differentiation and growth.

      ECMatrix™ consists of laminin, collagen type IV, heparan sulfate proteoglycans, entactin and nidogen. It also contains various growth factors (TGF-beta, FGF) and proteolytic enzymes (plasminogen, tPA, MMPs) that occur normally in EHS tumors. It is optimized for maximal tube-formation.

      Application:

      The CHEMICON In Vitro Angiogenesis Assay Kit represents a simple model of angiogenesis in which the induction or inhibition of tube formation by exogenous signals can be easily monitored. For assaying inhibitors or stimulators of tube formation, simply premix the endothelial cell suspension with different concentrations of the inhibitor or stimulator to be tested, before adding the cells to the top of the ECMatrixTM. The assay can be used to monitor the extent of tube assembly in various endothelial cells, e.g. human umbilical vein cells (HUVEC) or bovine capillary endothelial (BCE) cells.

      The CHEMICON In Vitro Angiogenesis Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.
      Materials Required but Not Delivered1. 96-well Tissue Culture plate

      2. Microcentrifuge Tubes, sterile

      3. 37°C Tissue Culture Incubator

      4. Inverted Light Microscope

      5. HUVEC cells or other experimental cell line (any species)
      References
      Product Information
      Components
      • ECMatrixTM Gel Solution: (Part No. 90060) Five 1 mL bottles.
      • ECMatrixTM Diluent Buffer, 10X: (Part No. 90061) One 1 mL bottle.
      Applications
      ApplicationThe In Vitro Angiogenesis Assay Kit represents a simple model of angiogenesis in which the induction or inhibition of tube formation by exogenous signals can be easily monitored.
      Key Applications
      • Activity Assay
      Biological Information
      Species Reactivity
      • Vertebrates
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore kit materials at -20°C for up to 6 months. Once ECMatrix™ has been thawed, store at 2-8°C for one week. Do not refreeze.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Antiangiogenic polyketides from Peperomia dindygulensis Miq.
      Qi-Wei Wang,De-Hong Yu,Meng-Gan Lin,Mei Zhao,Wen-Jun Zhu,Qin Lu,Gui-Xiu Li,Chao Wang,Yi-Fang Yang,Xue-Mei Qin,Chao Fang,Hong-Zhuan Chen,Guo-Hong Yang
      Molecules (Basel, Switzerland)  17  2012

      Show Abstract
      22504832 22504832
      Intrafibrillar silicification of collagen scaffolds for sustained release of stem cell homing chemokine in hard tissue regeneration.
      Li-Na Niu,Kai Jiao,Yi-Pin Qi,Sergey Nikonov,Cynthia K Y Yiu,Dwayne D Arola,Shi-Qiang Gong,Ahmed El-Marakby,Marcela R O Carrilho,Mark W Hamrick,Kenneth M Hargreaves,Anibal Diogenes,Ji-Hua Chen,David H Pashley,Franklin R Tay
      FASEB journal : official publication of the Federation of American Societies for Experimental Biology  26  2012

      Show Abstract
      22859369 22859369
      Silicate bioceramics induce angiogenesis during bone regeneration.
      Wanyin Zhai,Hongxu Lu,Lei Chen,Xiaoting Lin,Yan Huang,Kerong Dai,Kawazoe Naoki,Guoping Chen,Jiang Chang
      Acta biomaterialia  8  2012

      Show Abstract
      21964215 21964215
      Knock-down of endothelial connexins impairs angiogenesis.
      Christiane Gärtner,Barbara Ziegelhöffer,Martin Kostelka,Holger Stepan,Friedrich-Wilhelm Mohr,Stefan Dhein
      Pharmacological research : the official journal of the Italian Pharmacological Society  65  2012

      Show Abstract
      22154844 22154844
      Differential analysis of peripheral blood- and bone marrow-derived endothelial progenitor cells for enhanced vascularization in bone tissue engineering.
      Ami R Amini,Cato T Laurencin,Syam P Nukavarapu
      Journal of orthopaedic research : official publication of the Orthopaedic Research Society  30  2012

      Show Abstract
      22378621 22378621
      Plasma from the second and third weeks after open colorectal resection for cancer stimulates in vitro endothelial cell growth, migration, and invasion.
      H M C Shantha Kumara,Daniel Kirchoff,Samer Naffouje,Michael Grieco,Sonali A C Herath,Nadav Dujovny,Matthew F Kalady,Neil Hyman,Linda Njoh,Richard L Whelan,H M C Shantha Kumara
      Surgical endoscopy  26  2012

      Show Abstract
      22083320 22083320
      Overexpression of human papillomavirus (HPV) type 16 oncoproteins promotes angiogenesis via enhancing HIF-1α and VEGF expression in non-small cell lung cancer cells.
      Li G, He L, Zhang E, Shi J, Zhang Q, Le AD, Zhou K, Tang X
      Cancer letters  311  160-70. Epub 2011 Jul 22.  2011

      Show Abstract
      21868151 21868151
      Secolignans with Antiangiogenic Activities from Peperomia dindygulensis.
      Lin MG, Yu DH, Wang QW, Lu Q, Zhu WJ, Bai F, Li GX, Wang XW, Yang YF, Qin XM, Fang C, Chen HZ, Yang GH.
      Chemistry & biodiversity  8  862-71  2011

      Show Abstract
      21560234 21560234
      Isolation and expansion of functionally-competent cardiac progenitor cells directly from heart biopsies.
      Davis DR, Kizana E, Terrovitis J, Barth AS, Zhang Y, Smith RR, Miake J, Marbán E
      J Mol Cell Cardiol  49  312-21. Epub 2010 Mar 4.  2010

      Show Abstract Full Text Article
      20211627 20211627
      Sonic hedgehog paracrine signaling regulates metastasis and lymphangiogenesis in pancreatic cancer.
      J M Bailey,A M Mohr,M A Hollingsworth
      Oncogene  28  2009

      Show Abstract Full Text Article
      19633682 19633682

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      In Vitro Angiogenesis Assay Kit

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      Life Science Research > Cell Analysis > Cell-based Assays > Angiogenesis & Endothelial Transmigration Assays