402176 | ImmunoPen™

402176
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      View Pricing & Availability
      Click To Print This Page

      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      402176-1EA
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service

          Glass bottle 1 ea
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewDesigned to minimize waste of antibody in immunostaining techniques on paraffin sections, frozen sections, or cytology preparations. When applied in a circle around the specimen, forms a hydrophobic barrier, retaining antibody. Sufficient for 500-800 individual applications. Not available for sale in Japan.
          Catalogue Number402176
          Brand Family Calbiochem®
          References
          Product Information
          DeclarationNot available for sale in Japan.
          Applications
          Application NotesFrozen Sections
          Immunostaining Barrier
          Paraffin Sections
          Application CommentsTo start the ImmunoPen™ shake well and gently press the tip straight down several times until the fluid starts soaking the tip.

          Paraffin Section Method:
          Deparaffinize and rehydrate the section according to normal procedures. For best adhesion, dry around the section with tissue paper and encircle it with the ImmunoPen™. Allow the circle to dry for approximately 1-2 min at room temperature, and then return the slide to water or buffer. If trypsinization or other proteolytic digestion is required, apply the ImmunoPen™ barrier after the digestion is completed.
          Frozen Section Method:
          Apply the ImmunoPen™ circle after cleaning slides with xylene/ethanol or coating with egg albumin, glycerin, or poly-L-lysine. Apply before fixation or first immersion in water or buffer. If the slide is treated with a hydrophile, soak it in 0.1N hydrochloric acid for 20 s, then wash with water.
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +15°C to +30°C
          Do not freeze Ok to freeze
          Special InstructionsKeep cap tightly closed when pen is not in use, and store horizontally. Lasts for ~500-800 applications.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          402176

          Citations

          Title
        • Nürnberger, A., et al. 2004. J. Histochem. Cytochem. 52, 1647.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision06-August-2007 RFH
          ApplicationFrozen Sections
          Immunostaining Barrier
          Paraffin Sections
          DescriptionDesigned to minimize antibody waste in immunostaining techniques on paraffin sections, frozen sections, or cytology preparations. The pen contains liquid resistant to water, alcohol, and acetone. When applied in a circle around the specimen the liquid forms a hydrophobic barrier, retaining antibody and minimizing waste of valuable reagents. Multiple sections, separated by individ-ual circular barriers, can be applied to the same slide. The ImmunoPen™ can be used for immunostaining procedures employing the following methods: PAP (peroxidase-antiperoxidase), ABC, enzymatic, and frozen section. While the barrier is insoluble in alcohol and acetone, it can be removed by xylene after the staining procedure is complete.
          CommentsTo start the ImmunoPen™ shake well and gently press the tip straight down several times until the fluid starts soaking the tip.

          Paraffin Section Method:
          Deparaffinize and rehydrate the section according to normal procedures. For best adhesion, dry around the section with tissue paper and encircle it with the ImmunoPen™. Allow the circle to dry for approximately 1-2 min at room temperature, and then return the slide to water or buffer. If trypsinization or other proteolytic digestion is required, apply the ImmunoPen™ barrier after the digestion is completed.
          Frozen Section Method:
          Apply the ImmunoPen™ circle after cleaning slides with xylene/ethanol or coating with egg albumin, glycerin, or poly-L-lysine. Apply before fixation or first immersion in water or buffer. If the slide is treated with a hydrophile, soak it in 0.1N hydrochloric acid for 20 s, then wash with water.
          Storage +15°C to +30°C
          Do Not Freeze Ok to freeze
          Special InstructionsKeep cap tightly closed when pen is not in use, and store horizontally. Lasts for ~500-800 applications.
          Toxicity Standard Handling
          Citation
        • Nürnberger, A., et al. 2004. J. Histochem. Cytochem. 52, 1647.