EZHL-80SK Human Leptin "Dual Range" ELISA

EZHL-80SK
96-well strip plate  
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      Overview

      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      Leptin H ELISA Fluorescent, Colorimetric
      Description
      Catalogue NumberEZHL-80SK
      DescriptionHuman Leptin "Dual Range" ELISA
      Background InformationLeptin is synthesized in adipocytes as a 16 kDa molecule and the circulating level is directly proportional to the total amount of fat in the body.
      References
      Product Information
      Detection methodFluorescent Colorimetric
      Precision, %
      • Inter-assay: 2.6–6.2 Sensitive Assay 1.3–8.6
      • Intra-assay: 2.6–4.6 Sensitive Assay 1.4–4.9
      Applications
      ApplicationThis Human Leptin "Dual Range" ELISA is used to measure & quantify Leptin levels in Metabolism & Endocrine & Neuroscience research.
      Key Applications
      • ELISA
      Application NotesRoom temperature, 3.5 hour assay 25 μL sample size (Sensitive Assay: 50μL)
      Biological Information
      Species Reactivity
      • Human
      Analytes Available
      • Leptin
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development.
      Gene Symbol
      • LEP
      • leptin
      • OB
      • obesity
      • OBS
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P41159 # May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
      SIZE: 167 amino acids; 18641 Da
      SUBUNIT: Interacts with SIGLEC6.
      SUBCELLULAR LOCATION: Secreted.
      DISEASE:SwissProt: P41159 # Defects in LEP may be a cause of autosomal recessive obesity [MIM:601665].
      SIMILARITY: SwissProt: P41159 ## Belongs to the leptin family.
      Accuracy
      • 94–114%
      • Sensitive Assay 86–102%
      Physicochemical Information
      Sensitivity
      • 0.5 ng/mL
      • Sensitive Assay 0.125 ng/mL
      Linearity of Dilution80–105% Sensitive Assay 96–125%
      Standard Curve Range
      • 0.5–100 ng/mL
      • Sensitive Assay 0.125–20 ng/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • For research use only. Not for use in diagnostic procedures.
      Storage and Shipping Information
      Packaging Information
      Material Size96-well strip plate
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      Protocol: Human Leptin ELISA Kit- 96-Well Plate

      Human Leptin "Dual Range" ELISA SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Changes in glucose homeostasis after Roux-en-Y gastric bypass surgery for obesity at day three, two months, and one year after surgery: role of gut peptides.
      Falkén Y, Hellström PM, Holst JJ, Näslund E
      The Journal of clinical endocrinology and metabolism  96  2227-35. Epub 2011 May 4.  2011

      21543426 21543426
      A high-legume low-glycemic index diet reduces fasting plasma leptin in middle-aged insulin-resistant and -sensitive men.
      Zhang Z, Lanza E, Ross AC, Albert PS, Colburn NH, Rovine MJ, Bagshaw D, Ulbrecht JS, Hartman TJ
      Eur J Clin Nutr  65  415-8. Epub 2011 Jan 5.  2011

      Show Abstract
      21206508 21206508
      Role of baseline leptin and ghrelin levels on body weight and fat mass Changes after an energy-restricted diet intervention in
      Labayen I, Ortega FB, Ruiz JR, Lasa A, Simón E, Margareto J
      The Journal of clinical endocrinology and metabolism  96  E996-1000. Epub 2011 Apr 6.  2011

      21470990 21470990
      Is sprint exercise a leptin signaling mimetic in human skeletal muscle?
      Guerra B, Olmedillas H, Guadalupe-Grau A, Ponce-González JG, Morales-Alamo D, Fuentes T, Chapinal E, Fernández-Pérez L, De Pablos-Velasco P, Santana A, Calbet JA
      Journal of applied physiology (Bethesda, Md : 1985)  111  715-25. Epub 2011 Jun 9.  2011

      Show Abstract
      21659488 21659488
      Preoperative serum leptin levels in patients with breast cancer.
      Mehmet Aliustaoglu,Ahmet Bilici,Mahmut Gumus,Aslihan Topal Colak,Gulcan Baloglu,Rahmi Irmak,Mesut Seker,Bala Basak Oven Ustaalioglu,Tarik Salman,Berkant Sonmez,Taflan Salepci,Mustafa Yaylaci
      Medical oncology (Northwood, London, England)  27  2010

      Show Abstract
      19412673 19412673
      Metabolic state alters economic decision making under risk in humans.
      Symmonds, Mkael, et al.
      PLoS ONE, 5: e11090 (2010)  2010

      Show Abstract
      20585383 20585383
      Gender dimorphism in skeletal muscle leptin receptors, serum leptin and insulin sensitivity.
      Borja Guerra,Teresa Fuentes,Safira Delgado-Guerra,Amelia Guadalupe-Grau,Hugo Olmedillas,Alfredo Santana,Jesus Gustavo Ponce-Gonzalez,Cecilia Dorado,José A L Calbet
      PloS one  3  2008

      Show Abstract Full Text Article
      18941624 18941624
      A study on biomarkers, cytokines, and growth factors in children with burn injuries.
      N M Abdel-Hafez,Y Saleh Hassan,T H El-Metwally
      Annals of burns and fire disasters  20  2007

      Show Abstract Full Text Article
      21991076 21991076

      Technical Info

      Title
      96 well Template 2 Up
      96 well Template Single
      Quality Control Ranges: Human Leptin ELISA (Normal & Sensitive) Kit

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits