377853 Hirudin, Hirudo medicinalis, Recombinant, Saccharomyces cerevisiae - CAS 8001-27-2 - Calbiochem

377853
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      Overview

      Replacement Information

      Key Specifications Table

      CAS #
      8001-27-2

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      377853-2000U
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          Plastic ampoule 2000 u
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          Description
          OverviewHV1 form devoid of sulfate residue at Tyr63. Has highly specific antithrombin properties. Inactivates thrombin by blocking substrate binding groups.
          Catalogue Number377853
          Brand Family Calbiochem®
          References
          Product Information
          CAS number8001-27-2
          Unit of DefinitionOne unit is defined as the amount of hirudin that will neutralize 1 NIH unit of thrombin (fibrinogen assay) at 37°C, pH 7.5.
          ATP CompetitiveN
          FormLyophilized
          ReversibleN
          Applications
          Biological Information
          Primary TargetHas highly specific antithrombin properties
          Specific Activity≥10,000 antithrombin units (ATU)/mg protein
          Physicochemical Information
          Cell permeableN
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSMR8440000
          Safety Information
          R PhraseR: 20/21/22

          Harmful by inhalation, in contact with skin and if swallowed.
          S PhraseS: 36

          Wear suitable protective clothing.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Harmful
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          377853
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision12-May-2008 RFH
          DescriptionHV1 form devoid of the sulfate residue at Tyr63. Exhibits highly specific antithrombin properties. Inactivates thrombin by blocking substrate binding groups.
          FormLyophilized
          Recommended reaction conditions
          Protocol for the Assay of Hirudin Reagents: 1. 100 NIH units/ml thrombin 2. 9 mg/ml isotonic saline (0.9%) 3. 0.5% fibrinogen in isotonic saline 4. hirudin reconstituted with 1.0 ml of distilled H2O, isotonic saline, or other suitable buffer Assay: 1. Add 10-100 µl of the sample to be assayed to 200 µl of 0.5% fibrinogen solution and mix well. 2. Add 5 µl aliquots of a standardized thrombin solution (100 NIH units/ml) at one min intervals with mixing at 20°C. 3. The end-point is reached when a fibrin clot appears within one minute. Calculate the number of antithrombin units (ATU) of hirudin present in the solution. Note: This protocol is meant to serve only as a guideline. It may need to be modified for specific applications.
          CAS number8001-27-2
          RTECSMR8440000
          Specific activity≥10,000 antithrombin units (ATU)/mg protein
          Unit definitionOne unit is defined as the amount of hirudin that will neutralize 1 NIH unit of thrombin (fibrinogen assay) at 37°C, pH 7.5.
          SolubilityH₂O. Reconstitute with 1 ml H₂O.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Toxicity Harmful
          Merck USA index14, 4718