374805 Hemocyanin, Keyhole Limpet, Megathura crenulata

374805
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      374805-1GM
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          Plastic ampoule 1 gm
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          374805-250MG
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              Plastic ampoule 250 mg
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              Description
              OverviewStable solid containing more than 60% protein. Protein is 90% undissociated hemocyanin.
              Protein:≥60%; copper to protein ratio: ≥1.5 x 10-3
              Catalogue Number374805
              Brand Family Calbiochem®
              SynonymsKLH
              References
              ReferencesHayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol. 63, 37.
              Shuler, K.R., et al. 1992. J. Immunol. Methods 156, 137.
              Erlanger, B.F. 1980. Methods Enzymol. 70, 85.
              Product Information
              CAS number9013-72-3
              FormBlue to blue-gray lyophilized solid
              FormulationLyophilized from BES buffer, MgSO₄.
              PreservativeNone
              Applications
              ApplicationHemocyanin, Keyhole Limpet, Megathura crenulata, CAS 9013-72-3, is a large, multi-subunit, oxygen-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.
              Application CommentsProtein concentration: ≥60%
              Copper: ≥0.09%
              Copper to Protein ratio: ≥1.5 x 10-3
              Biological Information
              Purity≥90% by size exclusion chromatography
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Standard Handling
              Storage +2°C to +8°C
              Do not freeze Ok to freeze
              Special InstructionsDissolve at 5-10 mg/ml (protein) in PBS or other physiological buffer by GENTLE addition of the buffer (e.g. slowly added down the side of the tube). DO NOT vortex or agitate vigorously to dissolve product. (KLH is an extremely large protein complex and has a tendency to aggregate and precipitate from solutions.) For further dilutions, use an appropriate buffer and maintain the magnesium concentration at ≥10 mM. Solutions should be stored in the refrigerator (4°C).
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              SDS

              Title

              Safety Data Sheet (SDS) 

              Certificates of Analysis

              TitleLot Number
              374805

              References

              Reference overview
              Hayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol. 63, 37.
              Shuler, K.R., et al. 1992. J. Immunol. Methods 156, 137.
              Erlanger, B.F. 1980. Methods Enzymol. 70, 85.

              Brochure

              Title
              Keyhole Limpet Hemocyanin
              Data Sheet

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision10-March-2011 JSW
              SynonymsKLH
              DescriptionHemocyanin purified from Keyhole Limpet (Mega thura crenulata). Protein:≥60%; copper to protein ratio: ≥1.5 x 10-3
              BackgroundKeyhole limpet hemocyanin (KLH), a carrier protein with a molecular weight ranging from 3,000,000-7,500,000, has many available primary amines to facilitate protein conjugation. KLH is used to immunize animals to elicit antibodies to small molecules (haptens) by covalent conjugation.
              FormBlue to blue-gray lyophilized solid
              FormulationLyophilized from BES buffer, MgSO₄.
              CAS number9013-72-3
              Purity≥90% by size exclusion chromatography
              PreservativeNone
              CommentsProtein concentration: ≥60%
              Copper: ≥0.09%
              Copper to Protein ratio: ≥1.5 x 10-3
              Storage +2°C to +8°C
              Do Not Freeze Ok to freeze
              Special InstructionsDissolve at 5-10 mg/ml (protein) in PBS or other physiological buffer by GENTLE addition of the buffer (e.g. slowly added down the side of the tube). DO NOT vortex or agitate vigorously to dissolve product. (KLH is an extremely large protein complex and has a tendency to aggregate and precipitate from solutions.) For further dilutions, use an appropriate buffer and maintain the magnesium concentration at ≥10 mM. Solutions should be stored in the refrigerator (4°C).
              Toxicity Standard Handling
              Merck USA index14, 4647
              ReferencesHayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol. 63, 37.
              Shuler, K.R., et al. 1992. J. Immunol. Methods 156, 137.
              Erlanger, B.F. 1980. Methods Enzymol. 70, 85.

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              Life Science Research > Protein Detection and Quantification > Immunodetection Support Products > Adjuvants & Immunization Tools