368044 | Granzyme A, Human, Recombinant, E. coli

368044
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      368044-10UG
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      Stocked 
      Discontinued
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      Available
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          Plastic ampoule 10 μg
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          Description
          OverviewRecombinant, human granzyme A expressed in E. coli. Granzyme A is a trypsin-like serine protease secreted by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. A proapoptotic enzyme that induces cell death through caspase-independent mechanism, causing single-stranded DNA nicking and other apoptotic features including nuclear fragmentation, chromatin condensation, loss of mitochondrial potential, and membrane blebbing. Granzyme A is also known to bind to and cleave nucleolin and activate the thrombin receptor on neuronal cells and astrocytes. It also cleaves and activates interleukin-1β. Note: The M.W. is that of a homodimer.
          Catalogue Number368044
          Brand Family Calbiochem®
          SynonymsGzmA
          References
          ReferencesFan, Z., et al. 2002. Mol. Cell. Biol. 22, 2810.
          Russell, J.H., and Ley, T. J. 2002. Annu. Rev. Immunol. 20, 323.
          Beresford, P.J., et al. 2001. J. Biol. Chem. 276, 43285.
          Simon, M.M., and Kramer, M.D. 1994. Methods Enzymol. 244, 68.
          Product Information
          Unit of DefinitionOne unit is defined as the amount of enzyme that hydrolyzes 1 nmol Z-Lys-SBzl per min 25°C, pH 8.0.
          EC number3.4.21.78
          FormLiquid
          FormulationIn 520 mM NaCl, 50 mM MES, 10% glycerol, pH 6.0.
          Applications
          ApplicationGranzyme A, Human, Recombinant, E. coli, is a trypsin-like serine protease secreted by cytotoxic T cells and natural killer cells. Induces cell death through caspase-independent mechanism.
          Biological Information
          Specific Activity≥100 units/µg protein
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          368044

          References

          Reference overview
          Fan, Z., et al. 2002. Mol. Cell. Biol. 22, 2810.
          Russell, J.H., and Ley, T. J. 2002. Annu. Rev. Immunol. 20, 323.
          Beresford, P.J., et al. 2001. J. Biol. Chem. 276, 43285.
          Simon, M.M., and Kramer, M.D. 1994. Methods Enzymol. 244, 68.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision02-July-2008 RFH
          SynonymsGzmA
          DescriptionRecombinant, human granzyme A expressed in E. coli. Granzyme A is a trypsin-like serine protease found in the cytotoxic granules and secreted by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A is a pro-apoptotic enzyme that induces cell death through a caspase-independent mechanism. Granzyme A-induced apoptosis results in single-stranded DNA nicking, nuclear fragmentation, chromatin condensation, loss of mitochondrial potential, and membrane blebbing. Granzyme A is also known to bind to and cleave nucleolin, activate the thrombin receptor on neuronal cells and astrocytes, cleave and activate interleukin-1β, and cleave putative HLA-associated protein II (PHAP II). Recombinant human Granzyme A expressed in E. coli.
          FormLiquid
          FormulationIn 520 mM NaCl, 50 mM MES, 10% glycerol, pH 6.0.
          EC number3.4.21.78
          Specific activity≥100 units/µg protein
          Unit definitionOne unit is defined as the amount of enzyme that hydrolyzes 1 nmol Z-Lys-SBzl per min 25°C, pH 8.0.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesFan, Z., et al. 2002. Mol. Cell. Biol. 22, 2810.
          Russell, J.H., and Ley, T. J. 2002. Annu. Rev. Immunol. 20, 323.
          Beresford, P.J., et al. 2001. J. Biol. Chem. 276, 43285.
          Simon, M.M., and Kramer, M.D. 1994. Methods Enzymol. 244, 68.