345852 Geranylgeranyltransferase I, GST-Fusion, His•Tag®, Rat, Recombinant, E. coli

345852
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      345852-50UG
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          Plastic ampoule 50 μg
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          Description
          OverviewRecombinant, rat GGTase I composed of an α- and a β-subunit (74 and 43 kDa, respectively). The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag® sequence. Both the GST moiety and the His•Tag® sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved off by TEV protease. Requires Zn2+ for optimal activity.
          Catalogue Number345852
          Brand Family Calbiochem®
          SynonymsGGTase I
          References
          ReferencesKalinin, A., et al. 2001. Protein Expr. Purif. 22, 84.
          Zhang, F.L., et al. 1994. J. Biol. Chem. 269, 23465.
          Moomaw, J.F., and Casey, P.J., 1992. J. Biol. Chem. 267, 17438.
          Product Information
          FormLiquid
          FormulationIn 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.
          Applications
          ApplicationGeranylgeranyltransferase I, GST-Fusion, His•Tag®, Rat, Recombinant, E. coli, contains a 74 kDa α- & 43 kDa β-subunit. The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag.
          Biological Information
          Biological activity1 pmol enzyme will transfer ≥3 pmol geranylgeranyl to RhoA in 10 min at 37°C, pH 7.2.
          Purity≥90% by SDS-PAGE
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          345852

          References

          Reference overview
          Kalinin, A., et al. 2001. Protein Expr. Purif. 22, 84.
          Zhang, F.L., et al. 1994. J. Biol. Chem. 269, 23465.
          Moomaw, J.F., and Casey, P.J., 1992. J. Biol. Chem. 267, 17438.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision30-June-2008 RFH
          SynonymsGGTase I
          DescriptionRecombinant, rat GGTase I composed of an α- and a β-subunit (74 and 43 kDa, respectively). The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag® sequence. Both the GST moiety and the His•Tag® sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved by TEV protease. Requires Zn2+ for optimal activity. The enzyme is purified with Zn2+ tightly bound, so addition of Zn2+ to the reaction mix is not necessary unless further manipulation of the enzyme results in release of the ions.
          FormLiquid
          FormulationIn 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.
          Recommended reaction conditions50 mM HEPES, pH 7.2, 5 mM MgCl2, 1 mM DTT, 3 mM NP-40 alternative, 5.5 µM [3H]GG pyrophosphate, 30 pmol acceptor protein of choice, and desired amount of enzyme; incubate at 37°C for 20 min.; quench the reaction with 10% HCl in 90% Ethanol.
          Purity≥90% by SDS-PAGE
          Biological activity1 pmol enzyme will transfer ≥3 pmol geranylgeranyl to RhoA in 10 min at 37°C, pH 7.2.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesKalinin, A., et al. 2001. Protein Expr. Purif. 22, 84.
          Zhang, F.L., et al. 1994. J. Biol. Chem. 269, 23465.
          Moomaw, J.F., and Casey, P.J., 1992. J. Biol. Chem. 267, 17438.