361526 | GSK-3β, Rabbit Skeletal Muscle, Recombinant, E. coli

361526
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      361526-5KU
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      Stocked 
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          Plastic ampoule 5 ku
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          Description
          OverviewRecombinant, rabbit glycogen synthase kinase 3β expressed in E. coli. Dual specificity kinase. One of several protein kinases that phosphorylates glycogen synthase. Other substrates include p90rsk, Tau, c-Jun, and CREB. Plays a key role in Wnt and insulin signaling.
          Note: 1 KU = 1000 units.
          Catalogue Number361526
          Brand Family Calbiochem®
          SynonymsGSK 3β-Isozyme, Rabbit Skeletal Muscle, Recombinant, E. coli
          References
          ReferencesDajani, R., et al. 2001. Cell 105, 721.
          Wang, Q.M., et al. 1995. Biochem. Biophys. Res. Commun. 208, 796.
          Fiol, C.J., et al. 1994. J. Biol. Chem. 269, 32187.
          Wang, Q.M., et al. 1994. J. Biol. Chem. 269, 14566.
          Wang, Q.M., et al. 1994. Biochemistry 33, 143.
          deGroot, R.P., et al. 1993. Oncogene 8, 841.
          Ishiguro, K., et al. 1993. FEBS Lett. 325, 167.
          Product Information
          Activity≥250,000 units/ml
          Unit of DefinitionOne unit is defined as the amount of GSK-3 required to catalyze the transfer of 1 pmol phosphate to CREB phosphopeptide KRREILSRRPS(P)YRP (200 µM), in 1 min at 30°C in a reaction volume of 25 µl.
          FormLiquid
          FormulationIn 50 mM NaCl, 30 mM Tris-HCl, 5 mM DTT, 1 mM EDTA, 50% glycerol, 0.03% BRIJ®-35 Detergent, pH 7.5
          Applications
          ApplicationGSK-3β, Rabbit Skeletal MuscleHis-Tag®, Human, Recombinant, E. coli, is a dual specificity kinase that plays important roles in insulin- and Wnt-mediated signaling. It is inactivated by phosphorylation at Ser9.
          Biological Information
          Physicochemical Information
          ContaminantsDNase: <10% conversion of RFI DNA to RFII and linear forms. Phosphatases and Proteases: none detected. RNases: <10% RNA degradation.
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          361526

          References

          Reference overview
          Dajani, R., et al. 2001. Cell 105, 721.
          Wang, Q.M., et al. 1995. Biochem. Biophys. Res. Commun. 208, 796.
          Fiol, C.J., et al. 1994. J. Biol. Chem. 269, 32187.
          Wang, Q.M., et al. 1994. J. Biol. Chem. 269, 14566.
          Wang, Q.M., et al. 1994. Biochemistry 33, 143.
          deGroot, R.P., et al. 1993. Oncogene 8, 841.
          Ishiguro, K., et al. 1993. FEBS Lett. 325, 167.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision13-September-2007 RFH
          SynonymsGSK 3β-Isozyme, Rabbit Skeletal Muscle, Recombinant, E. coli
          DescriptionRecombinant, rabbit glycogen synthase kinase 3β expressed in E. coli. Glycogen synthase kinase 3β is a monomeric protein whose activity is independent of second messengers such as cAMP or IP3. Recent studies demonstrate that, like the dual specificity MAP kinase, it autophosphorylates on Ser, Thr, and Tyr. It phosphorylates several exogenous substrates, including protein phosphatase 1, the regulatory domain of protein kinase A, myelin basic protein, protein phosphatase inhibitor 2, c-jun, c-myb, c-myc, CREB, CREM, p90rsk, and microtubule-associated tau protein only on Ser and/or Thr, but not on Tyr residues. Plays a key role in Wnt and insulin signaling.
          FormLiquid
          FormulationIn 50 mM NaCl, 30 mM Tris-HCl, 5 mM DTT, 1 mM EDTA, 50% glycerol, 0.03% BRIJ®-35 Detergent, pH 7.5
          Recommended reaction conditions20 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 5 mM DTT, 200 µM ATP, 30°C. Optimal incubation times and enzyme concentrations must be determined empirically for each substrate.
          ContaminantsDNase: <10% conversion of RFI DNA to RFII and linear forms. Phosphatases and Proteases: none detected. RNases: <10% RNA degradation.
          Activity≥250,000 units/ml
          Unit definitionOne unit is defined as the amount of GSK-3 required to catalyze the transfer of 1 pmol phosphate to CREB phosphopeptide KRREILSRRPS(P)YRP (200 µM), in 1 min at 30°C in a reaction volume of 25 µl.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesDajani, R., et al. 2001. Cell 105, 721.
          Wang, Q.M., et al. 1995. Biochem. Biophys. Res. Commun. 208, 796.
          Fiol, C.J., et al. 1994. J. Biol. Chem. 269, 32187.
          Wang, Q.M., et al. 1994. J. Biol. Chem. 269, 14566.
          Wang, Q.M., et al. 1994. Biochemistry 33, 143.
          deGroot, R.P., et al. 1993. Oncogene 8, 841.
          Ishiguro, K., et al. 1993. FEBS Lett. 325, 167.