344291 | Freund’s Incomplete Adjuvant, Modified

344291
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      Overview

      Replacement Information

      Key Specifications Table

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      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      344291-10ML
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          Glass bottle 10 ml
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          Description
          OverviewContains 85% Drakeol 5NF, 15% Arlacel A (mannide monooleate emulsifier). Heat sterilized. Contains zero colony forming units/ml.
          Catalogue Number344291
          Brand Family Calbiochem®
          References
          ReferencesFreund, J. 1947. Ann. Rev. Microbiol. 1, 291.
          Product Information
          FormYellow oily liquid
          Applications
          ApplicationFreund’s Incomplete Adjuvant, Modified Contains 85% Drakeol 5NF, 15% Arlacel A. Heat sterilized. Contains zero colony forming units.
          Application Comments10 ml of Freund's Incomplete Adjuvant homogenized in the following amounts by weight:

          85% Drakeol 5 NF (light mineral oil)
          15% Arlacel A, mannide monooleate emulsifier.

          1. Take the vial of Friend's Incomplete Adjuvant and fold the aluminum seal back to permit free access to the stopper. Be careful not to break the aluminum ring that holds the stopper in place.
          2. Dissolve 50 mg of antigen in 5.0 ml of sterile PBS, pH 7.0-7.3. Maintain or create sterility if at all feasible.
          3. Draw the solution into a 10 ml syringe with a 1 inch 21 gauge needle.
          4. Insert needle into adjuvant vial and forcibly drive solution into the vial. Withdraw about half the mixture into syringe and force it into the adjuvant vial again.
          5. Repeat the mixing procedure in step 4 until a smooth, stable, milky, water-in-oil emulsion forms. Check if the emulsion is complete by expelling a drop from the syringe onto the water surface in a beaker. If the drop remains on the surface and does not disperse when the beaker is agitated slightly, the emulsion is adequate. A properly prepared emulsion is stable for days.
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +15°C to +30°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          344291

          References

          Reference overview
          Freund, J. 1947. Ann. Rev. Microbiol. 1, 291.

          Brochure

          Title
          Keyhole Limpet Hemocyanin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision26-June-2008 RFH
          DescriptionThe word "adjuvant" is derived from the Latin, adjuvare, meaning "to help." During immunization, an adjuvant is generally mixed with the antigen to enhance the rate and amount of antibody formed. The most popular complete adjuvant is that of Freund. It consists of killed and dried microbial cells suspended in an emulsifying oil. Adjuvant without the microbial cells is called "incomplete adjuvant." Complete adjuvant forms a stable water-in-oil emulsion with aqueous solution of antigen. When injected subcutaneously into animals, the emulsified antigen forms a depot from which antigen is delivered slowly and at sustained levels to the animal's antibody-synthesizing system. The induced antibody response is usually high and sustained. In guinea pigs, an additional benefit from adjuvant is the delayed appearance of hypersensitivity.
          FormYellow oily liquid
          SolubilityComposition
          10 ml of Freund's Incomplete Adjuvant homogenized in the following amounts by weight:
          85% Drakeol 5 NF (light mineral oil)
          15% Arlacel A, mannide monooleate emulsifier

          Sample Preparation
          1. Take the vial of Friend's Incomplete Adjuvant and fold the aluminum seal back to permit free access to the stopper. Be careful not to break the aluminum ring that holds the stopper in place.
          2. Dissolve 50 mg of antigen in 5 ml sterile PBS, pH 7.0-7.3. Maintain or create sterility if at all feasible.
          3. Draw the solution into a 10 ml syringe with a 1 inch 21 gauge needle.
          4. Insert needle into adjuvant vial and forcibly drive solution into the vial. Withdraw about half the mixture into syringe and force it into the adjuvant vial again.
          5. Repeat the mixing procedure in step 4 until a smooth, stable, milky, water-in-oil emulsion forms. Check if the emulsion is complete by expelling a drop from the syringe onto the water surface in a beaker. If the drop remains on the surface and does not disperse when the beaker is agitated slightly, the emulsion is adequate. A properly prepared emulsion is stable for days.
          Comments10 ml of Freund's Incomplete Adjuvant homogenized in the following amounts by weight:

          85% Drakeol 5 NF (light mineral oil)
          15% Arlacel A, mannide monooleate emulsifier.

          1. Take the vial of Friend's Incomplete Adjuvant and fold the aluminum seal back to permit free access to the stopper. Be careful not to break the aluminum ring that holds the stopper in place.
          2. Dissolve 50 mg of antigen in 5.0 ml of sterile PBS, pH 7.0-7.3. Maintain or create sterility if at all feasible.
          3. Draw the solution into a 10 ml syringe with a 1 inch 21 gauge needle.
          4. Insert needle into adjuvant vial and forcibly drive solution into the vial. Withdraw about half the mixture into syringe and force it into the adjuvant vial again.
          5. Repeat the mixing procedure in step 4 until a smooth, stable, milky, water-in-oil emulsion forms. Check if the emulsion is complete by expelling a drop from the syringe onto the water surface in a beaker. If the drop remains on the surface and does not disperse when the beaker is agitated slightly, the emulsion is adequate. A properly prepared emulsion is stable for days.
          Storage +15°C to +30°C
          Do Not Freeze Ok to freeze
          Toxicity Standard Handling
          ReferencesFreund, J. 1947. Ann. Rev. Microbiol. 1, 291.