344145 | FPTase, Rat, Recombinant, E. coli

344145
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      344145-100UG
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          Plastic ampoule 100 μg
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          Description
          OverviewRecombinant, rat FPTase expressed in E. coli. A heterodimeric enzyme that catalyzes the transfer of a 15-carbon isoprenoid group to a variety of cellular proteins including Ras. Recognizes the C-terminal CAAX cysteine motif where the farnesylated cysteine is followed by two aliphatic amino acids and either leucine or serine.
          M.W. α-subunit: ~48,000; β-subunit: ~46,000
          Catalogue Number344145
          Brand Family Calbiochem®
          SynonymsFarnesyl Proteintransferase, FTase
          References
          ReferencesHightower, K.E., et al. 2001. Biochem. J. 360, 625.
          Zimmerman, K.K., et al. 1998. Protein Expr. Purif. 14, 395.
          Reiss, Y., et al. 1990. Cell 62, 81.
          Product Information
          FormLiquid
          FormulationIn 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.
          Applications
          ApplicationFPTase, Rat, Recombinant, E. coli, a heterodimeric enzyme catalyzes the transfer of a 15-carbon isoprenoid group to a variety of cellular proteins. Recognizes the C-terminal CAAX cysteine motif.
          Biological Information
          Biological activity1 pmol of FPTase will transfer 1 pmol of farnesyl to H-Ras in 15 min at 37°C.
          Purity≥85% by SDS-PAGE
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          344145

          References

          Reference overview
          Hightower, K.E., et al. 2001. Biochem. J. 360, 625.
          Zimmerman, K.K., et al. 1998. Protein Expr. Purif. 14, 395.
          Reiss, Y., et al. 1990. Cell 62, 81.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision05-October-2010 JSW
          SynonymsFarnesyl Proteintransferase, FTase
          DescriptionRecombinant, rat FPTase expressed in E. coli. A heterodimeric enzyme that catalyzes the transfer of a 15-carbon isoprenoid group to a C-terminal cysteine in a variety of cellular proteins, including Ras. Recognizes the CaaX motif where the farnesylated cysteine (C) is followed by two aliphatic amino acids (aa) and either leucine or serine (X). Expressed in E. coli as an untagged recombinant protein.
          FormLiquid
          FormulationIn 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.
          Purity≥85% by SDS-PAGE
          Biological activity1 pmol of FPTase will transfer 1 pmol of farnesyl to H-Ras in 15 min at 37°C.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesHightower, K.E., et al. 2001. Biochem. J. 360, 625.
          Zimmerman, K.K., et al. 1998. Protein Expr. Purif. 14, 395.
          Reiss, Y., et al. 1990. Cell 62, 81.