324845 Endothelial Cell Growth Supplement, Bovine Neural Tissue

324845
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      324845-15MG
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
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          Will advise
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          Plastic ampoule 15 mg
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          Description
          OverviewNative endothelial cell growth supplement from bovine neural tissue. A cell culture-medium supplement screened for contamination by bacteria, fungi, and mycoplasma. Mitogenic activity thought to be physically, chemically, and biologically distinct from that of FGF. Acts as a superior cloning factor for the isolation and growth of hybridoma cells and may eliminate the need for feeder cell layers.
          Catalogue Number324845
          Brand Family Calbiochem®
          SynonymsECGS
          References
          ReferencesBlann, A.D. 1991. Cell Biol. Int. Rep. 15, 493.
          Jassal, D., et al. 1991. In Vitro Cell. Dev. Biol. 27A, 625.
          Tanswell, A.K., et al. 1991. J. Dev. Physiol. 15, 199.
          Olander, J., et al. 1980. In Vitro 16, 209.
          Product Information
          FormLyophilized
          FormulationLyophilized from sterile-filtered NaCl containing streptomycin sulfate.
          Applications
          Biological Information
          Biological activitygrowth-promoting activity confirmed with fetal bovine endothelial cells
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          R PhraseR: 20/21/22-61

          Harmful by inhalation, in contact with skin and if swallowed.
          May cause harm to the unborn child.
          S PhraseS: 22-36/37/39-45

          Do not breathe dust.
          Wear suitable protective clothing, gloves and eye/face protection.
          In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Harmful & Carcinogenic / Teratogenic
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
          Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          324845

          References

          Reference overview
          Blann, A.D. 1991. Cell Biol. Int. Rep. 15, 493.
          Jassal, D., et al. 1991. In Vitro Cell. Dev. Biol. 27A, 625.
          Tanswell, A.K., et al. 1991. J. Dev. Physiol. 15, 199.
          Olander, J., et al. 1980. In Vitro 16, 209.

          Brochure

          Title
          Growth Factors and Cytokines Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision20-June-2008 RFH
          SynonymsECGS
          DescriptionNative endothelial cell growth supplement from bovine neural tissue. A cell-culture-medium supplement for the growth and maintenance of many cell types. Growth promoting activities have been described for human endothelial cells, bovine endothelial cells, and mouse Balb/c fibroblasts. Can also substitute for feeder cells in support of the clonal growth of hybridomas. Mitogenic activity of the preparation is thought to be physically, chemically, and biologically distinct from that of FGF. Media containing 50-150 μg/ml is recommended for culturing vascular endothelial cells. Acts as a superior cloning factor for the isolation and growth of hybridoma cells and may eliminate the need for feeder cell layers. Screened for contamination by bacteria, fungi, and mycoplasma.
          FormLyophilized
          FormulationLyophilized from sterile-filtered NaCl containing streptomycin sulfate.
          Concentration Label Please refer to vial label for lot-specific concentration
          Biological activitygrowth-promoting activity confirmed with fetal bovine endothelial cells
          SolubilityUsing aseptic technique, add 1-5 ml sterile serum-free medium and solubilize by gentle aspiration with a sterile pipette.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
          Toxicity Harmful & Carcinogenic / Teratogenic
          ReferencesBlann, A.D. 1991. Cell Biol. Int. Rep. 15, 493.
          Jassal, D., et al. 1991. In Vitro Cell. Dev. Biol. 27A, 625.
          Tanswell, A.K., et al. 1991. J. Dev. Physiol. 15, 199.
          Olander, J., et al. 1980. In Vitro 16, 209.