324708 | Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi

324708
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      324708-2UG
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          Plastic ampoule 2 μg
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          Description
          OverviewNative endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyzes proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
          Catalogue Number324708
          Brand Family Calbiochem®
          References
          ReferencesDrapeau, G.R. 1980. J. Biol. Chem. 255, 839.
          Product Information
          CAS number9001-92-7
          Unit of DefinitionOne unit is defined as the amount of enzyme that will release a sufficient amount of azo dye from AZOCOLL™ Substrate (Cat. No. 194932) to produce an increase in absorbance at 520 nm of 0.001 per min at 37°C, pH 7.5.
          EC number3.4.24.33
          FormWhite lyophilized solid
          FormulationLyophilized from 10 mM Tris-HCl, pH 7.5.
          Applications
          Biological Information
          Purity≥90% by SDS-PAGE
          Specific Activity≥20,000 units/mg protein
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          R PhraseR: 20-36/37/38-42/43

          Harmful by inhalation.
          Irritating to eyes, respiratory system and skin.
          May cause sensitization by inhalation and skin contact.
          S PhraseS: 22-26-36

          Do not breathe dust.
          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Harmful
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          324708

          References

          Reference overview
          Drapeau, G.R. 1980. J. Biol. Chem. 255, 839.

          Brochure

          Title
          Proteases Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision19-June-2008 RFH
          DescriptionNative endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyzes proteins and the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK.
          FormWhite lyophilized solid
          FormulationLyophilized from 10 mM Tris-HCl, pH 7.5.
          Recommended reaction conditions
          Protocol 1. Reconstitute in 50 µl of distilled H2O. 2. Dissolve the proteins to be sequenced in phosphate, acetate, or Tris buffer, pH 6.0-8.5. For proteins that are difficult to solubilize, add urea (up to 1 M), SDS (0.01%), or guanidine-HCl (up to 1 M) to the digestion buffer. 3. Use a 1:20-1:100 ratio of proteinase to protein by weight for sequence analysis. 4. Incubate between 2-18 h at 37°C. Incubation time is dependent on the amount of enzyme used.
          CAS number9001-92-7
          EC number3.4.24.33
          Purity≥90% by SDS-PAGE
          Specific activity≥20,000 units/mg protein
          Unit definitionOne unit is defined as the amount of enzyme that will release a sufficient amount of azo dye from AZOCOLL™ Substrate (Cat. No. 194932) to produce an increase in absorbance at 520 nm of 0.001 per min at 37°C, pH 7.5.
          SolubilityH₂O
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
          Toxicity Harmful
          ReferencesDrapeau, G.R. 1980. J. Biol. Chem. 255, 839.

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          Categories

          Life Science Research > Proteins and Enzymes > Other Enzymes