324726 | Endoglycosidase F2, Elizabethkingia meningosepticum, Recombinant, E. coli

324726
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      324726-200MIU
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          Plastic ampoule 200 miu
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          Description
          OverviewRecombinant, Elizabethkingia meningosepticum endoglycosidase F2 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and biantennary complex oligosaccharides from glycoproteins. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore is more suitable for deglycosylation of native proteins. This enzyme is not active above pH 6.0.
          Note: 1 mU = 1 milliunit.
          Catalogue Number324726
          Brand Family Calbiochem®
          SynonymsEndo-β-N-acetylglucosaminidase F2, Endo F2
          References
          ReferencesReddy, A., et al. 1998. Glycobiology 8, 633.
          Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
          Tarentino, A.L., et al. 1993. J. Biol. Chem. 268, 9702.
          Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.
          Product Information
          Activity≥5 units/ml
          Unit of DefinitionOne unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1 µmol porcine fibrinogen per min at 37°C, pH 4.5.
          EC number3.2.1.96
          FormLiquid
          FormulationIn 25 mM NaCl, 10 mM sodium acetate buffer, pH 4.5.
          Applications
          Biological Information
          Specific Activity≥20 units/mg protein
          Physicochemical Information
          ContaminantsN-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidases, proteases: none detected
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          324726

          References

          Reference overview
          Reddy, A., et al. 1998. Glycobiology 8, 633.
          Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
          Tarentino, A.L., et al. 1993. J. Biol. Chem. 268, 9702.
          Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision11-September-2007 RFH
          SynonymsEndo-β-N-acetylglucosaminidase F2, Endo F2
          DescriptionRecombinant, Elizabethkingia meningosepticum endoglycosidase F2 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and biantennary complex oligosaccharides from glycoproteins. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore, is more suitable for deglycosylation of native proteins.
          FormLiquid
          FormulationIn 25 mM NaCl, 10 mM sodium acetate buffer, pH 4.5.
          Recommended reaction conditions50 mM sodium acetate buffer, pH 4.5. (Note: enzyme is not active above pH 6.0)
          EC number3.2.1.96
          ContaminantsN-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidases, proteases: none detected
          Specific activity≥20 units/mg protein
          Activity≥5 units/ml
          Unit definitionOne unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1 µmol porcine fibrinogen per min at 37°C, pH 4.5.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesReddy, A., et al. 1998. Glycobiology 8, 633.
          Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
          Tarentino, A.L., et al. 1993. J. Biol. Chem. 268, 9702.
          Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.