324716 | Endo-α-N-acetylgalactosaminidase, Streptococcus pneumoniae, Recombinant, E. coli

324716
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      324716-50MIU
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          Plastic ampoule 50 miu
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          Description
          OverviewRecombinant, Streptococcus pneumoniae Endo-α-N-acetylgalactosaminidase expressed in E. coli. Catalyzes the hydrolysis of the unsubstituted Galβ1,3GalNAc core disaccharide attached to serine or threonine residues of glycopeptides and glycoproteins to afford free oligosaccharides. For carbohydrates containing sialic acid or fucose, pretreatment with neuraminidase or fucosidase is required.
          Note: 1 mU = 1 milliunit.
          Catalogue Number324716
          Brand Family Calbiochem®
          SynonymsO-Glycopeptide endo-D-galactosyl-N-acetyl-α-galactosaminohydrolase, O-Glycosidase
          References
          ReferencesWang, A.M., et al. 1998. Mol. Genet. Metab. 65, 165.
          Iwase, H., and Hotta, K. 1993. Methods Mol. Biol. 14, 151.
          Fan, J.Q., et al. 1990. Agric. Biol. Chem. 54, 233.
          Umemoto, J., et al. 1978. Anal. Biochem. 91, 186.
          Glasgow, L.R., et al. 1977. J. Biol. Chem. 252, 8615.
          Product Information
          CAS number59793-96-3
          Activity≥1 unit/ml
          Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-2-acetamido-2-deoxy-3-O-(β-D-galactopyranosyl)-α-D-galactopyranoside per min at 37°C, pH 5.0.
          EC number3.2.1.97
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, pH 7.5.
          Applications
          Biological Information
          Specific Activity≥10 units/mg protein
          Physicochemical Information
          ContaminantsN-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidase, proteases: none detected
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          324716

          References

          Reference overview
          Wang, A.M., et al. 1998. Mol. Genet. Metab. 65, 165.
          Iwase, H., and Hotta, K. 1993. Methods Mol. Biol. 14, 151.
          Fan, J.Q., et al. 1990. Agric. Biol. Chem. 54, 233.
          Umemoto, J., et al. 1978. Anal. Biochem. 91, 186.
          Glasgow, L.R., et al. 1977. J. Biol. Chem. 252, 8615.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision19-June-2008 RFH
          SynonymsO-Glycopeptide endo-D-galactosyl-N-acetyl-α-galactosaminohydrolase, O-Glycosidase
          DescriptionRecombinant, Streptococcus pneumoniae Endo-α-N-acetylgalactosaminidase expressed in E. coli. Catalyzes the hydrolysis of the unsubstituted Galβ1,3GalNAc core disaccharide attached to serine or threonine residues of glycopeptides and glycoproteins to afford free oligosaccharides.
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, pH 7.5.
          Recommended reaction conditions
          Endo-α-N-acetylgalactosaminidase, Streptococcus pneumoniae, Recombinant, E. coli Note: This protocol is provided only as a general guidline. Researchers should standardize this assay for their own specific needs and should consult published literature. Endo-α-N-acetylgalactosaminidase cleaves only unsubstituted Galβ1,3GalNAc disaccharides attached to the serine or threonine residues of glycoproteins or glycopeptides. Substitutions such as sialic acid, galactose, fucose, or N-acetylglucosamine must first be removed with the appropriate exoglycosidase prior to treatment with Endo-α-N-acetylgalactosaminidase. At a minimum, a neuraminidase (e.g. α2-3,6,8-Neuraminidase, Arthrobacter ureafaciens, Recombinant, E. coli, Cat. No. 480716) is almost always required to remove sialic acid. Required Reagents 250 µM substrate (p-nitrophenol-2-acetamido-2-deoxy-3-O-(β-Dgalactopyranosyl)-α-D-galactopyranoside (Cat. No. 575303; also called T antigen) in 5 mM Sodium phosphate, pH 5.0 1 M Na2CO3 Spectrophotometer Protocol 1. Adjust the spectrophotometer to read 400 nm. 2. Add 0.4 ml of substrate solution to two tubes and warm to 37°C. 3. Add 5 µl of enzyme to one tube and mix. Continue incubating at 37°C. 4. Following a 5 minute incubation, add 0.6 ml of 1 M Na2CO3 to both tubes. 5. Blank the spectrophotometer using the control tube without enzyme. 6. Read the absorbance of the sample at 400 nm. 7. Calculate the units: Units/ml = A/18.3 TV A = absorbance of the sample T = time in min V = volume of enzyme in ml 18.3 = millimolar extinction coefficient of pNP at pH >10 Note: Endo-α-N-acetylgalactosaminidase cleaves methylumbelliferyl-α-D-Nacetylgalactosamide (but not pNP-α-D-N-acetylgalactosamide) at 0.1% the rate of p-nitrophenyl-2-acetamido-2-deoxy-3-O-(β-D-galactopyranosyl)-α-D-galactopyranoside. Assay for Deglycosylation Required Reagents Endo-α-N-acetylgalactosaminidase 5X assay buffer: 250 mM sodium phosphate, pH 5.0 sterile dH2O α2-3,6,8-Neuraminidase, Arthrobacter ureafaciens, Recombinant, E. coli, (Cat. No. 480716) glycoprotein sample SDS-PAGE system Protocol 1. Add up to 100 µg of glycoprotein to a tube. 2. Add water to 13 µl and then add 4 µl of 5X assay buffer. 3. Add 1 µl of α2-3,6,8-Neuraminidase, Arthrobacter ureafaciens, Recombinant, E. coli. 4. Add 2 µl of Endo-α-N-acetylgalactosaminidase 5. Incubate at 37°C for 1 h. Cleavage may be monitored by SDS-PAGE if the size differential between the native and the de-O-glycosylated protein is sufficient for detection.
          CAS number59793-96-3
          EC number3.2.1.97
          ContaminantsN-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidase, proteases: none detected
          Specific activity≥10 units/mg protein
          Activity≥1 unit/ml
          Unit definitionOne unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-2-acetamido-2-deoxy-3-O-(β-D-galactopyranosyl)-α-D-galactopyranoside per min at 37°C, pH 5.0.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesWang, A.M., et al. 1998. Mol. Genet. Metab. 65, 165.
          Iwase, H., and Hotta, K. 1993. Methods Mol. Biol. 14, 151.
          Fan, J.Q., et al. 1990. Agric. Biol. Chem. 54, 233.
          Umemoto, J., et al. 1978. Anal. Biochem. 91, 186.
          Glasgow, L.R., et al. 1977. J. Biol. Chem. 252, 8615.