345811 | Endo-β-Galactosidase, Bacteroides fragilis, Recombinant, E. coli

345811
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      345811-50UL
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          Plastic ampoule 50 ul
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          Description
          OverviewRecombinant, Bacteroides fragilis endo-β-galactosidase expressed in E. coli. Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide.
          Catalogue Number345811
          Brand Family Calbiochem®
          References
          ReferencesMurata, T., et al. 2003. Eur. J. Biochem. 270, 3709.
          Scudder, P., et al. 1986. Eur. J. Biochem. 157, 365.
          Scudder, P., et al. 1984. J. Biol. Chem. 259, 6586.
          Scudder, P., et al. 1983. Biochem. J. 213, 485.
          Product Information
          Activity≥14 U/ml
          Unit of DefinitionOne unit is defined as the amount of enzyme that will liberate one µmol reducing sugar from bovine corneal keratan sulfate per min at 37°C, pH 5.8.
          EC number3.2.1.103
          FormLiquid
          FormulationSterile-filtered in 20 mM Tris-HCl, pH 7.5.
          Applications
          Biological Information
          Specific Activity≥140 U/mg protein
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsThere is no significant loss of activity if left at ambient temperature for several days. The enzyme is active for at least 5 days under the Recommended Reaction Conditions above.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          345811

          References

          Reference overview
          Murata, T., et al. 2003. Eur. J. Biochem. 270, 3709.
          Scudder, P., et al. 1986. Eur. J. Biochem. 157, 365.
          Scudder, P., et al. 1984. J. Biol. Chem. 259, 6586.
          Scudder, P., et al. 1983. Biochem. J. 213, 485.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision22-June-2007 JSW
          DescriptionRecombinant, Bacteroides fragilis endo-β-galactosidase expressed in E. coli. Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide. Optimum pH: 5.8.
          FormLiquid
          FormulationSterile-filtered in 20 mM Tris-HCl, pH 7.5.
          Recommended reaction conditions
          Activity Assay
          Required Reagents • 5X Reaction Buffer: 250 mM sodium phosphate, pH 5.8 Assay For Glycoproteins 1. Add up to 100 µg glycoprotein to a clean tube. 2. Add 4 µl 5X Reaction Buffer. 3. Add H2O to a total volume of 19 µl. 4. Add 1 µl endo-β-galactosidase. 5. Incubate at 37°C for 2 h. For Oligosaccharides Follow the assay as outlined above in steps 1-4. Incubate at 37°C for several h to several days, depending on the substrate.
          EC number3.2.1.103
          Specific activity≥140 U/mg protein
          Activity≥14 U/ml
          Unit definitionOne unit is defined as the amount of enzyme that will liberate one µmol reducing sugar from bovine corneal keratan sulfate per min at 37°C, pH 5.8.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsThere is no significant loss of activity if left at ambient temperature for several days. The enzyme is active for at least 5 days under the Recommended Reaction Conditions above.
          Toxicity Standard Handling
          ReferencesMurata, T., et al. 2003. Eur. J. Biochem. 270, 3709.
          Scudder, P., et al. 1986. Eur. J. Biochem. 157, 365.
          Scudder, P., et al. 1984. J. Biol. Chem. 259, 6586.
          Scudder, P., et al. 1983. Biochem. J. 213, 485.