324682 | Elastase, Porcine Pancreas, High Purity, Crystallized

324682
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      324682-1000U
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          Plastic ampoule 1000 u
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          324682-250U
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              Plastic ampoule 250 u
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              Description
              OverviewNative elastase from porcine pancreas. A serine protase that catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin and of a number of synthetic substrates containing aspartic acid, phenylalanine, or tyrosine. Inhibited by DFP, elastinal, and α2-macroglobulin.
              Catalogue Number324682
              Brand Family Calbiochem®
              References
              ReferencesLargman, C. et al. 1976. Biochemestry 15, 2491.
              Mandl, I. 1962. Methods in Enzymol. 5, 665.
              Product Information
              CAS number39445-21-1
              Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Suc-Ala-Ala-Pro-Abu-<i>p</i>NA (<a href = "/Products/ProductDisplay.asp→catNO=324699">Cat. No. 324699</a>) per min at 25°C, pH 8.0.
              EC number3.4.21.36
              FormLyophilized
              FormulationLyophilized from 50 mM Trehalose, 1 mM Acetic Acid.
              Applications
              Biological Information
              Specific Activity≥25 units/mg protein
              Physicochemical Information
              ContaminantsChymotrypsin: ≤0.2%
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              RTECSTS5052000
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Standard Handling
              Storage -20°C
              Protect from Moisture Protect from moisture
              Do not freeze Ok to freeze
              Special InstructionsFollowing reconstitution, elastase may be stored at 4°C at pH 6.0 for long term use. If incubated at room temperature at or near its optimum pH, elastase rapidly autolyzes to a mixture of peptides. Stock solutions are stable for up to 2 months at 4°C, pH 6.0.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              SDS

              Title

              Safety Data Sheet (SDS) 

              Certificates of Analysis

              TitleLot Number
              324682

              References

              Reference overview
              Largman, C. et al. 1976. Biochemestry 15, 2491.
              Mandl, I. 1962. Methods in Enzymol. 5, 665.

              Brochure

              Title
              Proteases Technical Bulletin
              Data Sheet

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision09-July-2010 JSW
              DescriptionNative elastase from porcine pancreas. Catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin, and of a number of synthetic substrates containing aspartic acid, glutamic acid, phenylalanine, or tyrosine. Preferentially cleaves peptide bonds at the carbonyl end of amino acid residues with small hydrophobic side chains, such as glycine, valine, leucine, isoleucine, and particularly alanine. Inhibited by DFP, elastinal, and α2-macroglobulin. Has an optimal pH of 7.8-8.5; pI = 9.5. Note: Elastase tends to adhere to glass. Hence, use of siliconized glassware is recommended.
              FormLyophilized
              FormulationLyophilized from 50 mM Trehalose, 1 mM Acetic Acid.
              CAS number39445-21-1
              RTECSTS5052000
              EC number3.4.21.36
              ContaminantsChymotrypsin: ≤0.2%
              Specific activity≥25 units/mg protein
              Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Suc-Ala-Ala-Pro-Abu-pNA (Cat. No. 324699) per min at 25°C, pH 8.0.
              SolubilitydH₂O (10 mg/ml)
              Storage -20°C
              Protect from moisture
              Do Not Freeze Ok to freeze
              Special InstructionsFollowing reconstitution, elastase may be stored at 4°C at pH 6.0 for long term use. If incubated at room temperature at or near its optimum pH, elastase rapidly autolyzes to a mixture of peptides. Stock solutions are stable for up to 2 months at 4°C, pH 6.0.
              Toxicity Standard Handling
              Merck USA index14, 3535
              ReferencesLargman, C. et al. 1976. Biochemestry 15, 2491.
              Mandl, I. 1962. Methods in Enzymol. 5, 665.

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              Categories

              Life Science Research > Proteins and Enzymes > Other Enzymes