324682 Elastase, Porcine Pancreas, High Purity, Crystallized

324682
  
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      Overview

      Replacement Information

      Key Specifications Table

      Description
      OverviewNative elastase from porcine pancreas. A serine protase that catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin and of a number of synthetic substrates containing aspartic acid, phenylalanine, or tyrosine. Inhibited by DFP, elastinal, and α2-macroglobulin.
      Catalogue Number324682
      Brand Family Calbiochem®
      References
      ReferencesLargman, C. et al. 1976. Biochemestry 15, 2491.
      Mandl, I. 1962. Methods in Enzymol. 5, 665.
      Product Information
      CAS number39445-21-1
      Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Suc-Ala-Ala-Pro-Abu-<i>p</i>NA (<a href = "/Products/ProductDisplay.asp→catNO=324699">Cat. No. 324699</a>) per min at 25°C, pH 8.0.
      EC number3.4.21.36
      FormLyophilized
      FormulationLyophilized from 50 mM Trehalose, 1 mM Acetic Acid.
      Applications
      Biological Information
      Specific Activity≥25 units/mg protein
      Physicochemical Information
      ContaminantsChymotrypsin: ≤0.2%
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSTS5052000
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Moisture Protect from moisture
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, elastase may be stored at 4°C at pH 6.0 for long term use. If incubated at room temperature at or near its optimum pH, elastase rapidly autolyzes to a mixture of peptides. Stock solutions are stable for up to 2 months at 4°C, pH 6.0.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Elastase, Porcine Pancreas, High Purity, Crystallized SDS

      Title

      Safety Data Sheet (SDS) 

      Elastase, Porcine Pancreas, High Purity, Crystallized Certificates of Analysis

      TitleLot Number
      324682

      References

      Reference overview
      Largman, C. et al. 1976. Biochemestry 15, 2491.
      Mandl, I. 1962. Methods in Enzymol. 5, 665.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-July-2010 JSW
      DescriptionNative elastase from porcine pancreas. Catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin, and of a number of synthetic substrates containing aspartic acid, glutamic acid, phenylalanine, or tyrosine. Preferentially cleaves peptide bonds at the carbonyl end of amino acid residues with small hydrophobic side chains, such as glycine, valine, leucine, isoleucine, and particularly alanine. Inhibited by DFP, elastinal, and α2-macroglobulin. Has an optimal pH of 7.8-8.5; pI = 9.5. Note: Elastase tends to adhere to glass. Hence, use of siliconized glassware is recommended.
      FormLyophilized
      FormulationLyophilized from 50 mM Trehalose, 1 mM Acetic Acid.
      CAS number39445-21-1
      RTECSTS5052000
      EC number3.4.21.36
      ContaminantsChymotrypsin: ≤0.2%
      Specific activity≥25 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Suc-Ala-Ala-Pro-Abu-pNA (Cat. No. 324699) per min at 25°C, pH 8.0.
      SolubilitydH₂O (10 mg/ml)
      Storage -20°C
      Protect from moisture
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, elastase may be stored at 4°C at pH 6.0 for long term use. If incubated at room temperature at or near its optimum pH, elastase rapidly autolyzes to a mixture of peptides. Stock solutions are stable for up to 2 months at 4°C, pH 6.0.
      Toxicity Standard Handling
      Merck USA index14, 3535
      ReferencesLargman, C. et al. 1976. Biochemestry 15, 2491.
      Mandl, I. 1962. Methods in Enzymol. 5, 665.

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      Categories

      Life Science Research > Proteins and Enzymes > Other Enzymes